Photodynamic therapy agent with a built-in apoptosis sensor for evaluating its own therapeutic outcome in situ
| Autor: | Klara Stefflova, Juan Chen, Hui Li, Diane Marotta, Gang Zheng |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chlorophyll
Programmed cell death medicine.medical_treatment Fluorescence spectrometry Photodynamic therapy Apoptosis Cell Line Tumor Drug Discovery medicine Humans Photosensitizer Viability assay Caspase Photosensitizing Agents biology Chemistry Caspase 3 Solutions Biochemistry Photochemotherapy Caspases Cancer cell Cancer research biology.protein Molecular Medicine Oligopeptides |
| Zdroj: | Journal of medicinal chemistry. 49(13) |
| ISSN: | 0022-2623 |
| Popis: | Identifying the extent of apoptosis in cells or tissues after cancer therapy in real time would be a powerful firsthand tool for assessing therapeutic outcome. We combined therapeutic and imaging functions in one agent, choosing photodynamic therapy (PDT) as an appropriate cancer treatment modality. This agent induces photodamage in irradiated cells and simultaneously identifies apoptotic cells by near-infrared fluorescence. This photodynamic therapy agent with a built-in apoptosis sensor (PDT-BIAS) contains a fluorescent photosensitizer used as an anticancer drug, connected to a fluorescence quencher by a caspase-3 cleavable peptide linker. We demonstrated that cleavage of the peptide linker by caspase-3, one of the executioner caspases involved in apoptosis, results in a detectable increase of fluorescence in solution and in cancer cells after PDT treatment. The apoptosis involvement and drug effectiveness were confirmed by Apoptag and cell viability (MTT) assays supporting the ability of PDT-BIAS to induce and image apoptosis in situ. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|