Eliciting cytotoxic T lymphocytes against human laryngeal cancer-derived antigens: evaluation of dendritic cells pulsed with a heat-treated tumor lysate and other antigen-loading strategies for dendritic-cell-based vaccination
| Autor: | Yi Wei, Yi Hui Wen, Thian-Sze Wong, Jia Wei Wei, Wei Gao, Wei Sun, Fan Qin Wei, Wei Ping Wen |
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| Rok vydání: | 2016 |
| Předmět: |
Cell Extracts
0301 basic medicine Cancer Research T cell medicine.medical_treatment Antigen presentation Cell Culture Techniques Antigen loading Tumor lysate Cancer Vaccines 03 medical and health sciences 0302 clinical medicine Antigen Laryngeal cancer Antigens Neoplasm Cell Line Tumor Tumor immunity Humans Medicine Cytotoxic T cell Antigen-presenting cell Laryngeal Neoplasms Antigen Presentation business.industry Research Dendritic Cells Immunotherapy Dendritic cell CTL 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Immunology Carcinoma Squamous Cell business T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Experimental & Clinical Cancer Research : CR |
| ISSN: | 1756-9966 |
| DOI: | 10.1186/s13046-016-0295-1 |
| Popis: | Background Dendritic cells (DCs) have been used successfully in clinical pilot studies. However, tumor-specific immunity and clinical responses were only induced in certain cancer patients. It has been well documented that immunotherapy efficacy can be optimized for responses using antigen pulsing. Methods The human laryngeal squamous cell cancer (LSCC) cell line SNU899 was used to evaluate the in vitro anti-tumor efficacy of three different preparations of dendritic cell (DC) vaccines consisting of either whole tumor cells or their derivatives including: i) DCs pulsed with a tumor cell supernatant (DC-TCS), ii) DCs pulsed with whole-cell tumor stressed lysate (DC-TSL), and iii) DCs pulsed with irradiated tumor cells (DC-ITC). Results Our results showed that DC-TSL is an effective source of tumor-associated antigens (TAAs) for pulsing DCs. DC-TSL induced the highest expansion of TAA-specific T cells, the strongest Th1 cytokine response, and the most potent cytotoxic T lymphocyte (CTL) activity. DC-TCS and DC-ITC inhibited T cell activation but induced a certain extent of CTL activity. Conclusions These data suggest that DC-TSL is a more potent inducer of antitumor immunity against laryngeal cancer than other antigen-loading strategies using whole tumor cell materials. This strategy provides an alternative approach for DC-based immunotherapy for laryngeal cancer. |
| Databáze: | OpenAIRE |
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