Use of a combination strategy to improve neuroprotection and neuroregeneration in a rat model of acute spinal cord injury
| Autor: | Adrian Flores-Romero, Estefanía E. de la Cruz-Castillo, Ivis Ibrahim Morales, Paola A. Santiago-Tovar, Cesar V. Borlongan, Emanuel Malagón-Axotla, Monserrat Rivas-Gonzalez, Marco A. Guerrero-Godinez, Vinnitsa Buzoianu-Anguiano, Elisa García, Roxana Rodríguez-Barrera, Antonio Ibarra, Laura C. Castillo-Carvajal |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Combination therapy neural derived peptides fibrin glue mesenchymal stem cells glial scar protective autoimmunity neural regeneration Pharmacology Neuroprotection lcsh:RC346-429 Glial scar 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Protective autoimmunity Medicine Spinal cord injury lcsh:Neurology. Diseases of the nervous system business.industry Mesenchymal stem cell medicine.disease Spinal cord Neuroregeneration 030104 developmental biology medicine.anatomical_structure business 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research, Vol 14, Iss 6, Pp 1060-1068 (2019) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.250627 |
| Popis: | Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strategies as a potential therapy for spinal cord injury. Immunization with neural derived peptides (INDP), inhibition of glial scar formation (dipyridyl: DPY), as well as the use of biocompatible matrix (fibrin glue: FG) impregnated with bone marrow mesenchymal stem cells (MSCs) were combined and then its beneficial effects were evaluated in the induction of neuroprotection and neuroregeneration after acute SCI. Sprague-Dawley female rats were subjected to a moderate spinal cord injury and then randomly allocated into five groups: 1) phosphate buffered saline; 2) DPY; 3) INDP + DPY; 4) DPY+ FG; 5) INDP + DPY + FG + MSCs. In all rats, intervention was performed 72 hours after spinal cord injury. Locomotor and sensibility recovery was assessed in all rats. At 60 days after treatment, histological examinations of the spinal cord (hematoxylin-eosin and Bielschowsky staining) were performed. Our results showed that the combination therapy (DPY+ INDP + FG + MSCs) was the best strategy to promote motor and sensibility recovery. In addition, significant increases in tissue preservation and axonal density were observed in the combination therapy group. Findings from this study suggest that the combination theapy (DPY+ INDP + FG + MSCs) exhibits potential effects on the protection and regeneration of neural tissue after acute spinal cord injury. All procedures were approved by the Animal Bioethics and Welfare Committee (approval No. 178544; CSNBTBIBAJ 090812960) on August 15, 2016. |
| Databáze: | OpenAIRE |
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