Usefulness of pan-fungal NASBA test for surveillance of environmental fungal contamination in a protected hematology unit
| Autor: | Hervé Pelloux, Thomas Berendsen, Gautier Szymanski, Claude Mabilat, Messalia Beghri, Sébatien Bailly, Anne Thiebaut-Bertrand, Hafid Abaibou, Frederick Lasnet, Marie-Pierre Brenier-Pinchart |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiological Techniques
medicine.medical_specialty Hematology Fungal contamination Fungi Hospital Departments General Medicine Biology NASBA Microbiology Infectious Diseases Molecular Diagnostic Techniques Internal medicine parasitic diseases Epidemiological Monitoring medicine Environmental Microbiology Animals Humans Self-Sustained Sequence Replication |
| Zdroj: | Medical mycology. 52(4) |
| ISSN: | 1460-2709 |
| Popis: | A pan-fungal nucleic acid sequence based applification (NASBA) test was adapted and used for the first time to detect and quantify the level of filamentous fungi in environmental samples. Surface samples (n = 356) collected in a controlled air flow hematology ward were tested by mycological culture and the pan-fungal NASBA test. The overall percentage of agreement between culture and NASBA was 88%, the Kappa coefficient was equal to 0.61 (95%CI = [0.51; 0.72]). This pan-fungal NASBA test could be a promising tool to rapidly monitor the absence of molds in controlled environments. |
| Databáze: | OpenAIRE |
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