Visualization and molecular characterization of whole-brain vascular networks with capillary resolution
| Autor: | Shota Morikawa, Hiroki R. Ueda, Ai Nakashima, Haruki Takeuchi, Etsuo A. Susaki, Shun Yamaguchi, Yuji Ikegaya, Takeyuki Miyawaki |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Fluorescence-lifetime imaging microscopy Science General Physics and Astronomy Signal-To-Noise Ratio Article Fluorescence imaging General Biochemistry Genetics and Molecular Biology Polymerization Bile Acids and Salts Mice 03 medical and health sciences Cerebral circulation Imaging Three-Dimensional 0302 clinical medicine 3-D reconstruction Animals lcsh:Science Fluorescent Dyes Histocytological Preparation Techniques Luminescent Agents Multidisciplinary Tissue clearing Chemistry Vascular connectivity Brain Hydrogels Vascular casting General Chemistry Cerebrovascular Circulation Capillaries Visualization Perfusion Luminescent Proteins Cross-Linking Reagents 030104 developmental biology Microscopy Fluorescence Self-healing hydrogels lcsh:Q Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Structural elucidation and molecular scrutiny of cerebral vasculature is crucial for understanding the functions and diseases of the brain. Here, we introduce SeeNet, a method for near-complete three-dimensional visualization of cerebral vascular networks with high signal-to-noise ratios compatible with molecular phenotyping. SeeNet employs perfusion of a multifunctional crosslinker, vascular casting by temperature-controlled polymerization of hybrid hydrogels, and a bile salt-based tissue-clearing technique optimized for observation of vascular connectivity. SeeNet is capable of whole-brain visualization of molecularly characterized cerebral vasculatures at the single-microvessel level. Moreover, SeeNet reveals a hitherto unidentified vascular pathway bridging cerebral and hippocampal vessels, thus serving as a potential tool to evaluate the connectivity of cerebral vasculature. Structural and molecular elucidation of cerebrovascular network is promising for understanding energy supply system in the brain. Here, the authors describe labeling and tissue clearing techniques that visualize the whole-brain vasculature in a molecularly characterizable manner. |
| Databáze: | OpenAIRE |
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