Hydrazinocurcumin Induces Apoptosis of Hepatocellular Carcinoma Cells Through the p38 MAPK Pathway

Autor: Kuangyuan Qiao, Chao Wang, Li Peng, Zhilei Zhang, Chong Zhang, Hong-Tao He, Yuming Jia, Wuhan Yang, Zhuo Xu
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
MAPK/ERK pathway
030213 general clinical medicine
Carcinoma
Hepatocellular
Curcumin
MAP Kinase Signaling System
Pyridines
p38 mitogen-activated protein kinases
RM1-950
p38 Mitogen-Activated Protein Kinases
030226 pharmacology & pharmacy
General Biochemistry
Genetics and Molecular Biology
Article
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
medicine
Animals
Humans
General Pharmacology
Toxicology and Pharmaceutics
Cell Proliferation
Cell growth
Chemistry
General Neuroscience
Research
Liver Neoplasms
Imidazoles
Hep G2 Cells
General Medicine
Articles
medicine.disease
Xenograft Model Antitumor Assays
digestive system diseases
Rats
Hydrazines
Toxicity Tests
Subacute
Apoptosis
Hepatocellular carcinoma
Cancer research
Therapeutics. Pharmacology
Public aspects of medicine
RA1-1270
Function (biology)
Zdroj: Clinical and Translational Science
Clinical and Translational Science, Vol 14, Iss 5, Pp 2075-2084 (2021)
ISSN: 1752-8062
1752-8054
Popis: Hydrazinocurcumin (HZC), a synthetic derivative of curcumin (CUR), has been documented to show anticancer potential in impeding tumor growth in several cancers, including hepatocellular carcinoma (HCC). However, the underlying molecular mechanisms remain unclear. This study aimed to explore the function and underlying mechanisms of HZC on HCC cells, which may involve the p38 mitogen activated protein kinase (MAPK) pathway. HZC was first purified and identified. HepG2 cells were then subjected to treatment with HZC or CUR of different concentrations and p38 MAPK signaling inhibitor (SB203580) to verify their effects on HCC cell apoptosis and proliferation. Furthermore, the functional relevance between HZC and the p38 MAPK pathway in HCC was examined. It was observed that 40 μM HZC exhibited the best pro‐apoptosis effect in HCC cells. HZC was found to inhibit HCC cell proliferation and promote apoptosis, the effect of which was stronger than 5‐fluorouracil (5‐FU). More importantly, the anti‐oncogenic effect of HZC and 5‐FU was implicated with activation of the p38 MAPK pathway. In vivo experimental results showed that HZC inhibited tumor growth more effectively than 5‐FU through the p38 MAPK pathway. These results provide evidence that HZC exerted anti‐oncogenic and pro‐apoptosis effects in HCC cells through activation of the p38 MAPK pathway.
Databáze: OpenAIRE
Externí odkaz: