Distinguishing self from nonself: immunogenicity of the murine H47 locus is determined by a single amino acid substitution in an unusual peptide
| Autor: | Peter A. Eden, Derry C. Roopenian, Lisa M. Mendoza, Gilbert Villaflor, Nilabh Shastri |
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| Rok vydání: | 2001 |
| Předmět: |
Minor Histocompatibility Loci
Phenylalanine Immunology Molecular Sequence Data chemical and pharmacologic phenomena Peptide Locus (genetics) Autoantigens Cell Line Minor Histocompatibility Antigens Mice Antigen MHC class I Minor histocompatibility antigen Immunology and Allergy Animals Humans Amino Acid Sequence Isoleucine Peptide sequence Alleles Genetics chemistry.chemical_classification Mice Inbred BALB C Polymorphism Genetic biology Immunogenicity Membrane Proteins Molecular biology Peptide Fragments Histocompatibility Mice Inbred C57BL chemistry Amino Acid Substitution biology.protein Mutagenesis Site-Directed Oligopeptides Protein Binding |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 166(7) |
| ISSN: | 0022-1767 |
| Popis: | Histocompatibility (H) Ags are responsible for chronic graft rejection and graft vs host disease in solid tissue and bone marrow transplantation among MHC-matched individuals. Here we defined the molecular basis of self-nonself discrimination for the murine chromosome 7 encoded H47 histocompatibility locus, known by its trait of graft-rejection for over 40 years. H47 encodes a novel, highly conserved cell surface protein containing the SCILLYIVI (SII9) nonapeptide in its transmembrane region. The p7 isoleucine-to-phenylalanine substitution in SII9 defined the antigenic polymorphism and T cell specificity. Despite absence of the canonical consensus motif and weak binding to Db MHC I, both H47 peptides were presented to CTLs. However, unlike all the other known H loci, the relative immunogenicity of both H47 alleles varied dramatically and was profoundly influenced by neighboring H loci. The results provide insights into the peptide universe that defines nonself and the basis of histoincompatibility. |
| Databáze: | OpenAIRE |
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