Comparison of sulfur transferases in various tissue and mitochondria of rats with type 1 diabetes mellitus induced by streptozotocin
| Autor: | Sevtap Bakir, Veysel Kenan Celik, Huseyin Aydin, Yusuf Kenan Tekin, Özlem Demirpençe, Ismail Sari |
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| Přispěvatelé: | [Aydin, Huseyin -- Celik, Veysel Kenan -- Sari, Ismail -- Tekin, Yusuf Kenan -- Demirpence, Ozlem -- Bakir, Sevtap] Cumhuriyet Univ, Sch Med, Dept Biochem, TR-58140 Sivas, Turkey, Sari, Ismail -- 0000-0003-3732-2102 |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Endocrinology Diabetes and Metabolism Type 1 diabetes mellitus Sulfurtransferase Mitochondrion Rhodanese 03 medical and health sciences Diabetes mellitus Internal medicine Internal Medicine Medicine Mercaptopyruvate sulfurtransferase Type 1 diabetes Kidney Lung business.industry medicine.disease Streptozotocin 030104 developmental biology medicine.anatomical_structure Endocrinology business medicine.drug |
| Zdroj: | International Journal of Diabetes in Developing Countries. 36:4-9 |
| ISSN: | 1998-3832 0973-3930 |
| DOI: | 10.1007/s13410-015-0377-1 |
| Popis: | WOS: 000373106100002 This study aims to investigate the relationship between sulfurtransferase (STS) activities [rhodanese (TST), mercaptopyruvate sulfurtransferase (MST)] involved in the catalysis of several biochemical reactions including detoxification of cyanide (CN-), restructuring of Fe-S cluster in proteins, and detoxification of oxygen radicals. Rats with type 1 diabetes mellitus induced by streptozotocin (STZ) were anesthetized at 14th day, and liver, lung, kidney, and heart tissues were extracted. All samples were homogenized, and mitochondrial parts were separated. Same processes were performed also in the control group, and TST and MST activities were measured in each part. The homogenate MST (MST (Homo) .) activities of the type 1 diabetes mellitus group were compared with the control group, and a decrease was observed in the lung, liver, and kidney, respectively; at the same time, an increase was seen in the heart tissue. The mitochondrial MST (MST (Mito) .) activities of rats with type 1 diabetes mellitus group were compared with the control group, and a decrease was found in all tissues. The highest decrease in the TST (Mito) . level of rats with type 1 diabetes mellitus was observed in kidney tissue. The TST activities of the type 1 diabetes mellitus group were compared with the control group, and a decrease was observed in the liver, lung, and kidney, respectively; at the same time, an increase was seen in the heart tissue. It is demonstrated in the present study that decreases occur both in enzyme levels of tissue homogenates and in mitochondria, of rats with induced type 1 diabetes mellitus. However, these results were not statistically significant. In the presence of these findings, we think that kidney, liver, lung, and heart tissue can be affected by type 1 diabetes in the long term. |
| Databáze: | OpenAIRE |
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