The heme auxotroph Caenorhabditis elegans can cleave the thioether bonds of c-type cytochromes
| Autor: | Despoina A. I. Mavridou, Stuart J. Ferguson, Jonathan Hodgkin, Annie C. Murphey |
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| Přispěvatelé: | Medical Research Council |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Biochemistry & Molecular Biology Hemeprotein Auxotrophy BIOGENESIS Biophysics AMINO-ACID-SEQUENCE Biochemistry MATURATION 03 medical and health sciences chemistry.chemical_compound Thioether Structural Biology BINDING Genetics thioether bond Animals Peptide bond BIOSYNTHESIS Caenorhabditis elegans Proteins Caenorhabditis elegans heme Molecular Biology Heme CHAPERONE Science & Technology 030102 biochemistry & molecular biology biology ACTIVE-SITE Cytochrome c Cytochromes c 0601 Biochemistry And Cell Biology Cell Biology biology.organism_classification heme recycling 0304 Medicinal And Biomolecular Chemistry CCME 030104 developmental biology cytochrome c chemistry Covalent bond ESCHERICHIA-COLI biology.protein heme source Life Sciences & Biomedicine SYSTEM |
| Popis: | Heme is essential and synthesised via highly-regulated processes. For this reason, most organisms strive to recycle it or acquire it from their environment. When heme is bound to proteins non-covalently, degradation of the polypeptide is sufficient to release it. However, in some hemoproteins, such as c-type cytochromes, heme is covalently bound to the protein backbone. We use the heme auxotroph Caenorhabditis elegans to investigate if cytochromes c can be a heme source, and we show that this organism must encode a novel system, which specifically cleaves the thioether bonds of c-type cytochromes. We also find that at limiting heme concentrations, while somatic tissues develop normally the germline fails to proliferate, suggesting the presence of a heme-sensing checkpoint in C. elegans. |
| Databáze: | OpenAIRE |
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