Upregulation of MDR- and EMT-Related Molecules in Cisplatin-Resistant Human Oral Squamous Cell Carcinoma Cell Lines

Autor: Hyeong Sim Choi, Pil-Young Yun, Young-Kyun Kim
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Epithelial-Mesenchymal Transition
MDR1
Antineoplastic Agents
Drug resistance
Rhodamine 123
Catalysis
Article
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line
Tumor
medicine
ATP Binding Cassette Transporter
Subfamily G
Member 2
Humans
MTT assay
Viability assay
ATP Binding Cassette Transporter
Subfamily B
Member 1
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Cisplatin
Organic Chemistry
EMT
General Medicine
Computer Science Applications
Neoplasm Proteins
Up-Regulation
Multiple drug resistance
Blot
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Cell culture
Drug Resistance
Neoplasm
030220 oncology & carcinogenesis
Cancer research
Carcinoma
Squamous Cell
BCRP
Mouth Neoplasms
OSCC
medicine.drug
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 12
International Journal of Molecular Sciences, Vol 20, Iss 12, p 3034 (2019)
ISSN: 1422-0067
Popis: Cisplatin is one of the major drugs used in oral cancer treatments, but its usage can be limited by acquired drug resistance. In this study, we established three cisplatin-resistant oral squamous cell carcinoma (OSCC) cell lines and characterized them using cell viability assays, qPCR, Western blotting, FACS, immunofluorescence, and wound healing assays. Three OSCC cell lines (YD-8, YD-9, and YD-38) underwent long-term exposure to cisplatin, eventually acquiring resistance to the drug, which was confirmed by an MTT assay. In these three newly established cell lines (YD-8/CIS, YD-9/CIS, and YD-38/CIS), overexpression of multidrug resistance (MDR)-related genes was detected by qPCR and Western blotting. The cell lines displayed an increase in the functional activities of breast cancer resistance protein (BCRP) and multidrug resistance protein1 (MDR1) by rhodamine 123 and bodipy FL prazosin accumulation assays. Moreover, the cisplatin-resistant cells underwent morphological changes, from round to spindle-shaped, increased expression of epithelial-to-mesenchymal transition (EMT)-related molecules such as N-cadherin, and showed increased cell migration when compared with the parental cell lines. These results suggest that these newly established cell lines have acquired drug resistance and EMT induction.
Databáze: OpenAIRE
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