Infrared neural stimulation induces intracellular Ca2+release mediated by phospholipase C
| Autor: | Claire Lefort, Rodney P. O'Connor, David Moreau, Philippe Lévêque, Jolien Pas, Sylvia M. Bardet |
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| Přispěvatelé: | Photonique Fibre et Sources Cohérentes (XLIM-PHOT), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Département Bioélectronique (BEL-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-CMP-GC, BIO-INGENIERIE (XLIM-BIO-INGENIERIE) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell signaling Cell type infrared neural stimulation General Physics and Astronomy IP3 signaling Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Calcium imaging Live cell imaging photobiomodulation General Materials Science phospholipase C Neural cell Phospholipase C Endoplasmic reticulum General Engineering General Chemistry Cell biology calcium imaging 030104 developmental biology [SDV.IB]Life Sciences [q-bio]/Bioengineering 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Journal of Biophotonics Journal of Biophotonics, Wiley, 2017, ⟨10.1002/jbio.201700020⟩ |
| ISSN: | 1864-063X 1864-0648 |
| DOI: | 10.1002/jbio.201700020 |
| Popis: | International audience; The influence of infrared laser pulses on intracellular Ca2+ signaling was investigated in neural cell lines with fluorescent live cell imaging. The probe Fluo-4 was used to measure Ca2+ in HT22 mouse hippocampal neurons and nonelectrically excitable U87 human glioblastoma cells exposed to 50 to 500ms infrared pulses at 1470nm. Fluorescence recordings of Fluo-4 demonstrated that infrared stimulation induced an instantaneous intracellular Ca2+ transient with similar dose-response characteristics in hippocampal neurons and glioblastoma cells (half-maximal effective energy density EC50 of around 58J.cm-2 ). For both type of cells, the source of the infrared-induced Ca2+ transients was found to originate from intracellular stores and to be mediated by phospholipase C and IP3 -induced Ca2+ release from the endoplasmic reticulum. The activation of phosphoinositide signaling by IR light is a new mechanism of interaction relevant to infrared neural stimulation that will also be widely applicable to nonexcitable cell types. The prospect of infrared optostimulation of the PLC/IP3 cell signaling cascade has many potential applications including the development of optoceutical therapeutics. |
| Databáze: | OpenAIRE |
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