Calcium paradox in skeletal muscles: physiologic and microscopic observations
| Autor: | Marco Antonio Soza, Stirling Carpenter, George Karpati |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Physiology
Diaphragm chemistry.chemical_element Calcium In Vitro Techniques Dantrolene Sodium Cellular and Molecular Neuroscience Sarcolemma Physiology (medical) Isometric Contraction medicine Respiratory muscle Myocyte Animals Edetic Acid Calcium channel Muscles Rats Inbred Strains Anatomy Fibronectins Rats Microscopy Electron chemistry Biophysics Neurology (clinical) Laminin Contracture medicine.symptom Muscle contraction Muscle Contraction |
| Zdroj: | Musclenerve. 9(3) |
| ISSN: | 0148-639X |
| Popis: | Immersion of rat hemidiaphragms in Ca2+-free Krebs solution (KS) containing Ca2+ chelator in vitro leads to separation of basal lamina from the plasma membrane, as well as transient contracture and rapid loss of twitch response [calcium paradox (CP) phase 1]. Subsequent immersion in regular KS results in necrosis of muscle fibers accompanied by slowly increasing contracture (CP phase 2). This contracture could be prevented or reduced by using either Ca2+-free KS or calcium channel blockers, but not by dantrolene sodium, implying that after drastic reduction of extracellular and sarcolemmal Ca2+ during CP phase 1, the sarcolemma has lost its ability to control normal Ca2+ fluxes. Contracture did not develop at 21 degrees C. CP is a convenient model to study calcium-induced muscle cell death and the role of Ca2+ in maintaining sarcolemmal integrity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text