Protein Production and Crystallization at SECSG – An Overview

Autor: Irina Kataeva, Ting Li, Joseph Ng, Tamara A. Dailey, Dawei Lin, Robert J. Bunzel, M. Gary Newton, Bi-Cheng Wang, C. Shah, Lisa Nagy, F.J. Sugar, Han-Seung Lee, Farris L. Poole, Hao Xu, John Rose, Chi Hao Luan, Zhi-Jie Liu, Larry DeLucas, Peter S. Horanyi, Harry A. Dailey, M.R. Mayer, Francis E. Jenney, Songlin Li, J. Habel, A. Shah, Ming Luo, Michael W. W. Adams
Rok vydání: 2005
Předmět:
Zdroj: Journal of Structural and Functional Genomics. 6:233-243
ISSN: 1570-0267
1345-711X
Popis: Using a high degree of automation, the Southeast Collaboratory for Structural Genomics (SECSG) has developed high throughput pipelines for protein production, and crystallization using a two-tiered approach. Primary, or tier-1, protein production focuses on producing proteins for members of large Pfam families that lack a representative structure in the Protein Data Bank. Target genomes are Pyrococcus furiosus and Caenorhabditis elegans. Selected human proteins are also under study. Tier-2 protein production, or target rescue, focuses on those tier-1 proteins, which either fail to crystallize or give poorly diffracting crystals. This two tier approach is more efficient since it allows the primary protein production groups to focus on the production of new targets while the tier-2 efforts focus on providing additional sample for further studies and modified protein for structure determination. Both efforts feed the SECSG high throughput crystallization pipeline, which is capable of screening over 40 proteins per week. Details of the various pipelines in use by the SECSG for protein production and crystallization, as well as some examples of target rescue are described.
Databáze: OpenAIRE
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