Enhanced NTPDase and 5′-nucleotidase activities in diabetes mellitus and iron-overload model
| Autor: | Liliane de Freitas Bauermann, Gilberto Ignácio Lunkes, André L.B. Morsch, Rafael Fernandes Zanin, Vinícius Ribas Miron, Maísa Corrêa, Maria Rosa Chitolina Schetinger, Adriane Cismoski da Silva, Cinthia M. Mazzanti, Vera Maria Morsch |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Blood Glucose Blood Platelets Male medicine.medical_specialty Iron Overload Clinical chemistry Iron medicine.medical_treatment Clinical Biochemistry Ferric Compounds Diabetes Mellitus Experimental 5'-nucleotidase Hemoglobins chemistry.chemical_compound Antigens CD Adenine nucleotide Internal medicine Alloxan Diabetes mellitus medicine Animals Humans Platelet Rats Wistar 5'-Nucleotidase Molecular Biology Saline chemistry.chemical_classification Nitrates Adenine Nucleotides Hydrolysis Apyrase Cell Biology General Medicine medicine.disease Rats Disease Models Animal Endocrinology Enzyme Hematocrit chemistry |
| Zdroj: | Molecular and Cellular Biochemistry. 298:101-107 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/s11010-006-9357-6 |
| Popis: | The activity of the enzymes NTPDase and 5'-nucleotidase was studied in both diabetes mellitus and an associated model of iron-overload. Rats were divided in five groups: citrate (CC), saline (S), diabetic (D), iron-overload (IO), and diabetic iron-overload (DIO). Diabetes was induced with alloxan (150 mg/kg), and iron-overload was induced with iron-dextran (10 intramuscular applications of +/-80 mg/kg). The enzymatic activities were evaluated in the platelets. The results demonstrated an increase in the activity of NTPDase with substrates ATP and ADP (60% and 120%, respectively; P0.001), and 5'-nucleotidase (60%, P0.001). This increase was more intense in the IO and DIO groups. The results obtained in vitro showed an activation in ATP, ADP, and AMP hydrolysis between 1 microM and 1,000 microM ferric nitrate concentrations, being more pronounced at 100 microM and decreasing at 1,000 microM. We concluded that diabetes mellitus in association with iron-overload increased the hydrolysis of adenine nucleotides in platelets, contributing to the abnormalities found in these pathological conditions. |
| Databáze: | OpenAIRE |
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