Epidemiological analysis of Trueperella abortisuis isolated from cases of pig abortion of a single farm
| Autor: | Mazen Alssahen, Christoph Lämmler, Ellen Prenger-Berninghoff, Abdulwahed Ahmed Hassan, Markus Timke, Osama Sammra, Amir Abdulmawjood, Stefanie P. Glaeser, Jörn-Peter Wickhorst, Peter Kämpfer |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Farms Genotype Swine Sequence analysis Population Biology Microbiology DNA sequencing 03 medical and health sciences Pregnancy Germany RNA Ribosomal 16S Animals Genetic variability education Phylogeny 030304 developmental biology Swine Diseases Genetics 0303 health sciences education.field_of_study Phylogenetic tree 030306 microbiology Genetic Variation Sequence Analysis DNA General Medicine Abortion Veterinary 16S ribosomal RNA DNA Fingerprinting genomic DNA Phenotype Aborted Fetus Actinomycetaceae Female Actinomycetales Infections |
| Zdroj: | Folia Microbiologica. 65:491-496 |
| ISSN: | 1874-9356 0015-5632 |
| DOI: | 10.1007/s12223-019-00753-9 |
| Popis: | The present study was designed to characterize six Trueperella (T.) abortisuis strains, cultured over a period of 5 months from fetus and abortion material of six pigs of a single farm in Mecklenburg-West Pomerania federal state, Germany. It was of interest to investigate the epidemiological relationships of the six strains among each other and whether a single bacterial clone was responsible for the abortion situation of the single farm. All six strains were identified phenotypically, by MALDI-TOF MS analysis and by phylogenetic analysis based on 16S rRNA gene and gap (encoding the glyceraldehyde 3-phosphate dehydrogenase) and tuf (encoding elongation factor tu) gene sequencing. Further genotypic comparison was performed using different genomic DNA fingerprint methods including BOX-PCR, (GTG)5-PCR, and three RAPD-PCRs. The sequence analysis of the genes gap and tuf and the genomic DNA fingerprinting results revealed, as noval findings, that the six T. abortisuis strains cultured from a single farm represent six different bacterial clones showing a genetic variability of this bacterial species in the pig population. All six T. abortisuis strains were isolated in mixed culture with several other bacterial species. However, the T. abortisuis strain, generally found in high numbers, seemed to be responsible for the abortion situation in the farm. |
| Databáze: | OpenAIRE |
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