Overexpression of microRNAs miR-25-3p, miR-185-5p and miR-132-3p in Late Onset Fetal Growth Restriction, Validation of Results and Study of the Biochemical Pathways Involved
Autor: | Gabriela Loscalzo, Julia Scheel, José Santiago Ibañez-Cabellos, Eva García-Lopez, Shailendra Gupta, José Luis García-Gimenez, Salvador Mena-Mollá, Alfredo Perales-Marín, José Morales-Roselló |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
fetal health
QH301-705.5 late-onset fetal growth restriction Models Biological Article Catalysis miRNA-25-3p miRNA132-3p Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Humans Gene Regulatory Networks Biology (General) Physical and Theoretical Chemistry QD1-999 network analysis Molecular Biology Spectroscopy miRNA 030304 developmental biology cerebroplacental ratio 0303 health sciences Fetal Growth Retardation 030219 obstetrics & reproductive medicine Gene Expression Profiling Organic Chemistry miRNA185-5p Reproducibility of Results bioinformatics General Medicine Computer Science Applications Chemistry MicroRNAs Gene Ontology Gene Expression Regulation Signal Transduction |
Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 293 International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 23, Iss 293, p 293 (2022) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms23010293 |
Popis: | In a prospective study, 48 fetuses were evaluated with Doppler ultrasound after 34 weeks and classified, according to the cerebroplacental ratio (CPR) and estimated fetal weight (EFW), into fetuses with normal growth and fetuses with late-onset fetal growth restriction (LO-FGR). Overexpression of miRNAs from neonatal cord blood belonging to LO-FGR fetuses, was validated by real-time PCR. In addition, functional characterization of overexpressed miRNAs was performed by analyzing overrepresented pathways, gene ontologies, and prioritization of synergistically working miRNAs. Three miRNAs: miR-25-3p, miR-185-5p and miR-132-3p, were significantly overexpressed in cord blood of LO-FGR fetuses. Pathway and gene ontology analysis revealed over-representation of certain molecular pathways associated with cardiac development and neuron death. In addition, prioritization of synergistically working miRNAs highlighted the importance of miR-185-5p and miR-25-3p in cholesterol efflux and starvation responses associated with LO-FGR phenotypes. Evaluation of miR-25-3p; miR-132-3p and miR-185-5p might serve as molecular biomarkers for the diagnosis and management of LO-FGR; improving the understanding of its influence on adult disease. |
Databáze: | OpenAIRE |
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