A Trichophyton Rubrum Infection Model Based on the Reconstructed Human Epidermis - Episkin®
| Autor: | Jinling Yi, Congxiu Ye, Zhirui Chen, Wei Lai, Jian Chen, Xian-Yan Chen, Panpan Liang, Han Ma, Xin-Zhu Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology lcsh:Medicine Fungus Trichophyton rubrum macromolecular substances Conidium Microbiology Pathogenesis 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Stratum corneum medicine Trichophyton skin and connective tissue diseases Epidermis (botany) biology EpiSkin Infection Model Trichophyton Rubrum lcsh:R General Medicine biology.organism_classification bacterial infections and mycoses Staining medicine.anatomical_structure bacteria |
| Zdroj: | Chinese Medical Journal, Vol 129, Iss 1, Pp 54-58 (2016) |
| ISSN: | 0366-6999 |
| Popis: | Background: Trichophyton rubrum represents the most common infectious fungus responsible for dermatophytosis in human, but the mechanism involved is still not completely understood. An appropriate model constructed to simulate host infection is the prerequisite to study the pathogenesis of dermatophytosis caused by T. rubrum . In this study, we intended to develop a new T. rubrum infection model in vitro , using the three-dimensional reconstructed epidermis - EpiSkin ® , and to pave the way for further investigation of the mechanisms involved in T. rubrum infection. Methods: The reconstructed human epidermis (RHE) was infected by inoculating low-dose (400 conidia) and high-dose (4000 conidia) T. rubrum conidia to optimize the infection dose. During the various periods after infection, the samples were processed for pathological examination and scanning electron microscopy (SEM) observation. Results: The histological analysis of RHE revealed a fully differentiated epidermis with a functional stratum corneum, which was analogous to the normal human epidermis. The results of hematoxylin and eosin staining and the periodic acid-Schiff staining showed that the infection dose of 400 conidia was in accord with the pathological characteristics of host dermatophytosis caused by T. rubrum . SEM observations further exhibited the process of T. rubrum infection in an intuitionistic way. Conclusions: We established the T. rubrum infection model on RHE in vitro successfully. It is a promising model for further investigation of the mechanisms involved in T. rubrum infection. |
| Databáze: | OpenAIRE |
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