Recovery of a function involving gene duplication by retroposition in Saccharomyces cerevisiae

Autor: Jacky de Montigny, Yves Tourrette, Jean-Luc Souciet, Joseph Schacherer, Serge Potier
Přispěvatelé: Génétique moléculaire, génomique, microbiologie (GMGM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2004
Předmět:
MESH: Multie
Saccharomyces cerevisiae Proteins
Retroelements
Transcription
Genetic
Sequence analysis
MESH: Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
5' Flanking Region
5' flanking region
Saccharomyces cerevisiae
Genes
Fungal
Biology
MESH: Aspartate Carbamoyltransferase
MESH: 3' Flanking Region
Transcription (biology)
Multienzyme Complexes
Gene Duplication
Gene Expression Regulation
Fungal
Gene duplication
Genetics
Aspartate Carbamoyltransferase
3' Flanking Region
Letters
DNA
Fungal
Gene
Genetics (clinical)
Regulation of gene expression
MESH: 5' Flanking Region
Recombination
Genetic
[SDV.GEN]Life Sciences [q-bio]/Genetics
MESH: Gene Duplication
Fungal genetics
Chromosome Mapping
Sequence Analysis
DNA
biology.organism_classification
MESH: Chromosomes
Fungal
MESH: DNA
Fungal
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Chromosomes
Fungal
MESH: Chromosome Mapping
MESH: Genes
Fungal
MESH: Gene Expression Regulation
Fungal
Zdroj: Genome Research
Genome Research, Cold Spring Harbor Laboratory Press, 2004, 14 (7), pp.1291-7. ⟨10.1101/gr.2363004⟩
ISSN: 1088-9051
1549-5469
DOI: 10.1101/gr.2363004⟩
Popis: The duplication of DNA sequences contributes to genomic plasticity and is known to be one of the key factors responsible for evolution. The mechanisms underlying these rare events, which have been frequently mentioned by authors performing genomic analysis, have not yet been completely elucidated. These mechanisms were approached here in the yeast Saccharomyces cerevisiae, using a positive selection screen based on a particular mutated allele of the URA2 gene. Spontaneous revertants containing a duplication of the terminal part of the URA2 gene were selected and analyzed. Some important features of the duplicated regions, such as their chromosome location, size, and insertion sites, were characterized. The events selected correspond to a single inter- or intrachromosomal gene duplication process. The duplicated ATCase sequence is generally punctuated by a poly(A) tract and is always located in Ty1 sequences. In addition, the activation of a Ty1 transcription process increased the frequency of the duplication events. All in all, these data suggest that the duplication mechanism involves the reverse transcription of mRNA and the subsequent integration of the cDNA into a Ty1 area. The Ty1 elements and the retrotransposon-encoded function are key factors contributing to chromosomal reshaping. The genomic rearrangements described constitute experimental evidence for the recovery of a function involving duplication by retroposition.
Databáze: OpenAIRE
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