Differential Expression of Proteins From Cultured Endothelial Cells Exposed to Uremic Versus Normal Serum
| Autor: | Berta Fuste, Joaquín Abián, Montserrat Carrascal, Aleix Cases, Gemma Arderiu, Gines Escolar, Carla Carbo, Maribel Diaz-Ricart |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Proteomics Nephrology medicine.medical_specialty Inflammation medicine.disease_cause Internal medicine medicine Humans Endothelial dysfunction SOD Cells Cultured Uremia Kidney business.industry Endothelial Cells Middle Aged Blood Physiological Phenomena medicine.disease Endothelial stem cell Cross-Sectional Studies medicine.anatomical_structure Endocrinology Oxidative stress Protein Biosynthesis Chronic Disease Female Kidney Diseases medicine.symptom business Kidney disease |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0272-6386 |
| Popis: | [Background]: Deficient hemostasis and accelerated atherosclerosis coexist in patients with chronic kidney disease. Endothelial dysfunction may be involved in the high incidence of atherothrombotic events in these patients. We established an in vitro model of endothelial dysfunction by exposing endothelial cells to uremic media and applied a proteomic approach to characterize endothelial cell dysfunction in uremia. [Study Design]: Cross-sectional study. [Setting and Participants]: Serum samples from 8 patients with chronic kidney disease on hemodialysis treatment were collected. [Predictor]: Exposure of cultured endothelial cells to normal and uremic serum. [Outcome and Measurements]: Proteins from lysed cells were characterized by isoelectric point and molecular weight by using 2-dimensional gel electrophoresis. Spots were visualized by means of silver staining and identified by using mass spectrometry. [Results]: Identification of the most prominent proteins showed molecules related to inflammation (high mobility group box 1, aldose reductase, and proteasome components) and oxidative stress (superoxide dismutase and glutathione peroxidase), both associated with chronic kidney disease. These changes may be caused by activation of the nuclear factor-κB transcription factor. Changes in expression of cytoskeletal proteins (destrin and vimentin) also were detected. [Limitations]: In vitro study. [Conclusion]: Proteomic techniques proved to be a powerful tool to investigate endothelial dysfunction in uremia. A more exhaustive analysis will provide answers and potential therapeutic targets in the near future. This work was partially supported by grant SAF2006-08003 from the Ministerio de Ciencia y Tecnología; grants FIS PI060260, FIS PI040887, FIS CP04/00112, and FIS PI050153 from Fondo de Investigaciones de la Seguridad Social; and grant SGR2005-00952 from the Generalitat de Catalunya. Dr Carbo received grant 2006FI00092 from the Departament d’Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya and Fons Social Europeu. |
| Databáze: | OpenAIRE |
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