Development of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella abortisuis isolated from pigs

Autor:	Marwa F.E. Ahmed, Mazen Alssahen, Christoph Lämmler, Geoffrey Foster, Antonia Kreitlow, Isabel Hennig-Pauka, Madeleine Plötz, Amir Abdulmawjood
Rok vydání:	2021
Předmět:	Male Molecular Diagnostic Techniques Swine RNA Ribosomal 16S Actinomycetaceae Animals Female Cell Biology Molecular Biology Arcanobacterium Nucleic Acid Amplification Techniques Sensitivity and Specificity
Zdroj:	Molecular and cellular probes. 62
ISSN:	1096-1194
Popis:	The first description of Trueperella (T.) abortisuis was presented in Japan in 2009 by Azuma and colleagues. In the current study, eight T. abortisuis strains were identified by a newly developed loop-mediated isothermal amplification (LAMP) assay based on the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) encoding gene gap. Two T. abortisuis strains were obtained from prepuce of a seven-month-old boar and pooled foetal stomach contents in the United Kingdom, while the other six T. abortisuis strains were recovered from aborted foetal material of six pigs from a single farm in Germany. The developed LAMP assay showed an analytical sensitivity of 22 pg μL
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70aa4ed95e5e81497d34d31feaae76df https://pubmed.ncbi.nlm.nih.gov/35131429 Zobrazit plný text záznamu Full Text from ScienceDirect