Resting Escherichia coli as Chassis for Microbial Electrosynthesis: Production of Chiral Alcohols
| Autor: | Jeannine C. Mayr, Falk Harnisch, Lena Hartmann, Jan-Hendrik Grosch, Antje C. Spiess, Luis F. M. Rosa |
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| Rok vydání: | 2019 |
| Předmět: |
biology
Chemistry General Chemical Engineering Microbial electrosynthesis 02 engineering and technology Nicotinamide adenine dinucleotide 010402 general chemistry 021001 nanoscience & nanotechnology Electrosynthesis 01 natural sciences Combinatorial chemistry Cofactor 0104 chemical sciences chemistry.chemical_compound General Energy Biocatalysis biology.protein Environmental Chemistry General Materials Science NAD+ kinase 0210 nano-technology Enantiomeric excess Alcohol dehydrogenase |
| Zdroj: | ChemSusChem. 12:1631-1634 |
| ISSN: | 1864-564X 1864-5631 |
| DOI: | 10.1002/cssc.201900413 |
| Popis: | Chiral alcohols constitute important building blocks that can be produced enantioselectively by using nicotinamide adenine dinucleotide (phosphate) [NAD(P)H]-dependent oxidoreductases. For NAD(P)H regeneration, electricity delivers the cheapest reduction equivalents. Enzymatic electrosynthesis suffers from cofactor and enzyme instability, whereas microbial electrosynthesis (MES) exploits whole cells. Here, we demonstrate MES by using resting Escherichia coli as biocatalytic chassis for a production platform towards fine chemicals through electric power. This chassis was exemplified for the synthesis of chiral alcohols by using a NADPH-dependent alcohol dehydrogenase from Lactobacillus brevis for synthesis of (R)-1-phenylethanol from acetophenone. The E. coli strain and growth conditions affected the performance. Maximum yields of (39.4±5.7) % at a coulombic efficiency of (50.5±6.0) % with enantiomeric excess >99 % was demonstrated at a rate of (83.5±13.9) μm h-1 , confirming the potential of MES for synthesis of high-value compounds. |
| Databáze: | OpenAIRE |
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