Rapid and Efficient Genetic Transformation of Sorghum via Agrobacterium -Mediated Method
| Autor: | Hyeyoung Lee, Phat T. Do, Zhanyuan J. Zhang, Kimberly Nelson-Vasilchik, Albert P. Kausch |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Agrobacterium Transgene 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Transformation Genetic Sorghum Selectable marker biology business.industry fungi Gene Transfer Techniques food and beverages General Medicine Plants Genetically Modified biology.organism_classification Biotechnology Transformation (genetics) 030104 developmental biology Glufosinate chemistry Agrobacterium tumefaciens Callus business 010606 plant biology & botany Transformation efficiency |
| Zdroj: | Current Protocols in Plant Biology. 3:e20077 |
| ISSN: | 2379-8068 |
| DOI: | 10.1002/cppb.20077 |
| Popis: | Genetic transformation via Agrobacterium-mediated methodology has been used in many sorghum studies. However, the transformation efficiency still varies significantly due to high dependence on sorghum genotypes and technical expertise. In this article, we describe a sorghum transformation procedure in sufficient detail using a public genotype, P898012. This system utilizes a standard binary transgenic vector carrying the bar gene as a selectable marker and immature embryos as starting explants. Glufosinate is employed as the selective agent during callus and shoot induction. This procedure is relatively rapid, efficient, highly reproducible, and should be applicable for many other sorghum genotypes. © 2018 by John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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