A laboratory study of the effect of calcium hydroxide mixed with iodine or electrophoretically activated copper on bacterial viability in dentinal tubules
| Autor: | O. Cherniak, Ervin Itzchak Weiss, A. Mizrahi, Shaul Lin, Zvi Fuss |
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| Rok vydání: | 2002 |
| Předmět: |
Electrophoresis
Root canal Inorganic chemistry Smear layer Colony Count Microbial chemistry.chemical_element Enterococcus faecalis Agar plate Calcium Hydroxide chemistry.chemical_compound stomatognathic system medicine Animals General Dentistry Colony-forming unit Calcium hydroxide biology Root Canal Irrigants Potassium Iodide biology.organism_classification Copper Drug Combinations Dentinal Tubule medicine.anatomical_structure chemistry Dentin Anti-Infective Agents Local Cattle Dental Pulp Cavity Nuclear chemistry Iodine |
| Zdroj: | International endodontic journal. 35(6) |
| ISSN: | 0143-2885 |
| Popis: | AIM The aim of this laboratory study was to evaluate the ability of calcium hydroxide (CH), CH/iodine-potassium iodide (IKI) and electrophoretically activated copper to kill bacteria in dentinal tubules. METHODOLOGY In an in vitro model of dentinal tubule infection, 42 cylindrical root specimens, prepared from freshly extracted bovine teeth were used. After removal of the smear layer, intracanal dentinal tubules were infected with Enterococcus faecalis for 3 weeks. CH alone or preparations of CH with copper or IKI were placed in the root canal for 1 week. In specimens containing copper/CH, an electrophoretic current(5 mA/5 min) was applied using two electrodes follow-ing placement of the medicament in the canal. Powder dentine samples obtained from the canal wall using ISO sizes: 025, 027, 029, 031 and 033 burs were examined for the presence of viable bacteria by inoculating agar plates and counting colony forming units (cfu). RESULTS A significant difference was found between the experimental groups and the positive control group. CH and CH/IKI significantly (P < 0.001)reduced bacterial viability in dentinal tubules to a depth of 200 microm. Specimens with CH/IKI had significantly fewer viable bacteria than CH alone in tubules between the depths of 200-500 microm. Treatment with CH/copper and electrophoresis was most effective: specimens showed no viable bacteria in dentinal tubules to a depth of 500 microm from the root-canal space. CONCLUSIONS IKI or electrophoretically activated copper additives can significantly improve the antibacterial properties of CH in dentinal tubules. |
| Databáze: | OpenAIRE |
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