Vero microcultures for adenovirus neutralization tests

Autor:	J C Hierholzer, P G Bingham
Rok vydání:	1978
Předmět:	Microbiology (medical) Serotype Microculture Adenoviruses Human viruses Biology Antibodies Viral Virology Molecular biology Neutralization Cell Line Staining Titer Cytopathogenic Effect Viral Neutralization Tests Cell culture Helper virus Vero cell Research Article
Zdroj:	Journal of Clinical Microbiology. 7:499-506
ISSN:	1098-660X 0095-1137
DOI:	10.1128/jcm.7.6.499-506.1978
Popis:	A microculture neutralization test is described for measuring specific antibody levels to the 35 human adenovirus serotypes in Vero cells. The test is read at 5 days by macroscopic observation after staining the uninfected cells with crystal violet. The test is performed with a minimum of manipulations and gives serum titers comparable with those obtained in tube macrocultures of monkey kidney, human embryonic kidney, and Vero cells. The Vero microculture neutralization test measures inhibition of adenovirus toxicity, although selected human adenoviruses serially subpassaged in Vero cells were shown to successfully adapt and replicate in the absence of detectable helper viruses.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70a44a0523a24fc91f7b204b7e6d538c https://doi.org/10.1128/jcm.7.6.499-506.1978 Zobrazit plný text záznamu