Appearance pattern of TDP-43 in Japanese frontotemporal lobar degeneration with ubiquitin-positive inclusions
| Autor: | Michiko Minegishi, Takashi Togo, Koshiro Fujisawa, Kenji Kosaka, Hirotake Uchikado, Omi Katsuse, Heii Arai, Ryoko Yamamoto, Eizo Iseki, Shinji Higashi, Hiroaki Hino, Yoshiko Furukawa |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Neurite Cytoplasmic inclusion Inclusion bodies Degenerative disease Ubiquitin Asian People mental disorders medicine Humans Aged Aged 80 and over Inclusion Bodies biology General Neuroscience nutritional and metabolic diseases Brain Frontotemporal lobar degeneration Middle Aged medicine.disease Immunohistochemistry nervous system diseases DNA-Binding Proteins Cytoplasm biology.protein Dementia Female Immunostaining |
| Zdroj: | Neuroscience letters. 419(3) |
| ISSN: | 0304-3940 |
| Popis: | TAR-DNA-binding protein 43 (TDP-43) was identified as a major component of ubiquitin-positive intracellular inclusions from brains of patients with frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U). Here, we immunohistochemically investigated the appearance pattern of TDP-43 to compare the distribution of TDP-43-positive structures with that of ubiquitin-positive structures in brains of seven patients with Japanese FTLD-U, five of atypical Pick's disease (aPiD) and two of dementia with motor neuron disease (D-MND), as well as two patients with PiD as control. TDP-43-immunoreactivity generally colocalized to ubiquitin-immunoreactivity in both neuronal cytoplasmic inclusions and neurites in FTLD-U brains, but TDP-43-immunoreactivity alone or ubiquitin-immunoreactivity alone was also observed. In five aPiD cases, double-immunostaining with TDP-43 and ubiquitin demonstrated that diffuse neuronal cytoplasmic immunostaining for ubiquitin did not always display TDP-43-immunoreactivity. In contrast, ubiquitin-positive neuronal cytoplasmic inclusions usually displayed TDP-43-immunoreactivity in two D-MND cases, although most glial inclusions in one of two cases were immunostained only for TDP-43. TDP-43-positive structures were not detected in two PiD cases. Thus, the ratio in the appearance pattern of TDP-43 and ubiquitin was different between aPiD and D-MND, leading to the hypothesis that this difference may be associated with the two pathogenic variants related to clinical and pathological heterogeneity in FTLD-U. |
| Databáze: | OpenAIRE |
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