In vitro cultivation of canine multipotent mesenchymal stromal cells on collagen membranes treated with hyaluronic acid for cell therapy and tissue regeneration

Autor: Sergio Akinobu Yoshioka, P. R. O. Bersano, T. I. Wodewotzky, J. F. Lima-Neto, O. C. M. Pereira-Junior, Mateus José Sudano, S. A. F. Lima, Fernanda da Cruz Landim-Alvarenga
Přispěvatelé: Universidade Estadual Paulista (UNESP), Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp)
Rok vydání: 2012
Předmět:
medicine.medical_specialty
Physiology
Short Communication
Hyaluronic acid
medicine.medical_treatment
Cellular differentiation
Immunology
Basic fibroblast growth factor
Cell Culture Techniques
Cell- and Tissue-Based Therapy
Mesenchymal stromal cells
Biophysics
Biochemistry
Biomaterials
Cell therapy
chemistry.chemical_compound
Dogs
Tissue engineering
COLÁGENO
Animals
Regeneration
Medicine
General Pharmacology
Toxicology and Pharmaceutics
lcsh:QH301-705.5
Cell Proliferation
lcsh:R5-920
Collagen scaffolds
business.industry
General Neuroscience
Regeneration (biology)
Growth factor
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
General Medicine
Flow Cytometry
Immunohistochemistry
Cell biology
Surgery
lcsh:Biology (General)
chemistry
Cell culture
Collagen
lcsh:Medicine (General)
business
Zdroj: Brazilian Journal of Medical and Biological Research, Vol 45, Iss 12, Pp 1157-1162 (2012)
Brazilian Journal of Medical and Biological Research, Volume: 45, Issue: 12, Pages: 1157-1162, Published: DEC 2012
Brazilian Journal of Medical and Biological Research
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Brazilian Journal of Medical and Biological Research v.45 n.12 2012
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
SciELO
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2012007500149
Popis: Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:52:53Z No. of bitstreams: 1 S0100-879X2012001200008.pdf: 2235930 bytes, checksum: 352d1b6b4e48528e2848748d816a6f74 (MD5) Made available in DSpace on 2013-08-22T18:52:53Z (GMT). No. of bitstreams: 1 S0100-879X2012001200008.pdf: 2235930 bytes, checksum: 352d1b6b4e48528e2848748d816a6f74 (MD5) Previous issue date: 2012-12-01 Made available in DSpace on 2013-09-30T18:15:43Z (GMT). No. of bitstreams: 2 S0100-879X2012001200008.pdf: 2235930 bytes, checksum: 352d1b6b4e48528e2848748d816a6f74 (MD5) S0100-879X2012001200008.pdf.txt: 26410 bytes, checksum: 5cf09de1533d8848bd1ceda2b458c8e9 (MD5) Previous issue date: 2012-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:41:56Z No. of bitstreams: 2 S0100-879X2012001200008.pdf: 2235930 bytes, checksum: 352d1b6b4e48528e2848748d816a6f74 (MD5) S0100-879X2012001200008.pdf.txt: 26410 bytes, checksum: 5cf09de1533d8848bd1ceda2b458c8e9 (MD5) Made available in DSpace on 2014-05-20T13:41:56Z (GMT). No. of bitstreams: 2 S0100-879X2012001200008.pdf: 2235930 bytes, checksum: 352d1b6b4e48528e2848748d816a6f74 (MD5) S0100-879X2012001200008.pdf.txt: 26410 bytes, checksum: 5cf09de1533d8848bd1ceda2b458c8e9 (MD5) Previous issue date: 2012-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Support structures for dermal regeneration are composed of biodegradable and bioresorbable polymers, animal skin or tendons, or are bacteria products. The use of such materials is controversial due to their low efficiency. An important area within tissue engineering is the application of multipotent mesenchymal stromal cells (MSCs) to reparative surgery. The combined use of biodegradable membranes with stem cell therapy may lead to promising results for patients undergoing unsuccessful conventional treatments. Thus, the aim of this study was to test the efficacy of using membranes composed of anionic collagen with or without the addition of hyaluronic acid (HA) as a substrate for adhesion and in vitro differentiation of bone marrow-derived canine MSCs. The benefit of basic fibroblast growth factor (bFGF) on the differentiation of cells in culture was also tested. MSCs were collected from dog bone marrow, isolated and grown on collagen scaffolds with or without HA. Cell viability, proliferation rate, and cellular toxicity were analyzed after 7 days. The cultured cells showed uniform growth and morphological characteristics of undifferentiated MSCs, which demonstrated that MSCs successfully adapted to the culture conditions established by collagen scaffolds with or without HA. This demonstrates that such scaffolds are promising for applications to tissue regeneration. bFGF significantly increased the proliferative rate of MSCs by 63% when compared to groups without the addition of the growth factor. However, the addition of bFGF becomes limiting, since it has an inhibitory effect at high concentrations in culture medium. Universidade Estadual de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Reprodução Animal e Radiologia Veterinária Universidade Estadual de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Cirurgia e Anestesiologia Veterinária Universidade Estadual de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Patologia Veterinária Universidade de São Paulo Instituto de Química de São Carlos UNESP
Databáze: OpenAIRE
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