Correct in vivo processing of a chimeric ubiquitin-proapolipoprotein A-I fusion protein in baculovirus-infected insect cells
| Autor: | Francesca Varsalona, Michel Bulinckx, Nicole Moguilevsky, Alex Bollen, Jean-Paul Guillaume |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Baculoviridae
DNA Complementary viruses Recombinant Fusion Proteins Molecular Sequence Data Bioengineering Sf9 Spodoptera Moths Transfection Applied Microbiology and Biotechnology Cell Line Ubiquitin Complementary DNA parasitic diseases Polyhedrin Animals Humans Amino Acid Sequence Protein Precursors Peptide sequence Ubiquitins Apolipoproteins A biology Apolipoprotein A-I Base Sequence fungi General Medicine biology.organism_classification Fusion protein Molecular biology Recombinant Proteins Cell biology Molecular Weight biology.protein Electrophoresis Polyacrylamide Gel Lipoproteins HDL Protein Processing Post-Translational Biotechnology |
| Zdroj: | Journal of biotechnology. 32(1) |
| ISSN: | 0168-1656 |
| Popis: | The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product was purified and its N-terminal amino acid sequence was established. The data showed that authentic proapolipoprotein A-I has been produced, and thus demonstrated the existence in Spodoptera frugiperda insect cells of a specific ubiquitin hydrolase activity. |
| Databáze: | OpenAIRE |
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