Two tomato GDP-D-mannose epimerase isoforms involve in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development
| Autor: | Kentaro Mori, Jean-Claude Mollet, Christophe Rothan, Michel Hernould, Louise Mounet-Gilbert, Pierre Baldet, Joana Jorly, Martine Lemaire-Chamley, Carine Ferrand, Céline Bournonville, Arnaud Lehner, Marie Dumont, Antoine Monier, Isabelle Atienza, Patrice Lerouge, Rebecca Stevens |
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| Přispěvatelé: | Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité de recherche Génétique et amélioration des fruits et légumes (GALF), Normandie Université (NU) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine tomato fruits Physiology [SDV]Life Sciences [q-bio] Mannose Plant Science Ascorbic Acid 01 natural sciences chemistry.chemical_compound tomate Gene Expression Regulation Plant ascorbate cell wall GDP-D-mannose epimerase growth rhamnogalacturonan-II solanum lycopersicum 5-epimerase chemistry.chemical_classification Vegetal Biology rhamnogalacturonane food and beverages PMI: phosphomannose isomerase GMP: GDP-D-mannose pyrophosphorylase [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Agricultural sciences Isoenzymes tomato fruits AsA: ascorbic acid Biochemistry développement du fruit Pollen paroi cellulaire végétale Research Paper Gene isoform Germination AsA: ascorbic acid Biology Cell wall 03 medical and health sciences Biosynthesis gdp mannose épimérase [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Gene DPA: days post anthesis fungi biosynthèse de la paroi cellulaire Metabolism 030104 developmental biology Enzyme chemistry Carbohydrate Epimerases Function (biology) GME: GDP-D-mannose-3 Biologie végétale Sciences agricoles 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany 15 (67), 4767-4777. (2016) Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2016, 67 (15), pp.4767-4777. ⟨10.1093/jxb/erw260⟩ Journal of Experimental Botany, Oxford University Press (OUP), 2016, 67, pp.4767-4777. ⟨10.1093/jxb/erw260⟩ |
| ISSN: | 0022-0957 1460-2431 |
| DOI: | 10.1093/jxb/erw260⟩ |
| Popis: | Highlight The two tomato GDP-D-mannose epimerase isoforms play specific roles in cell wall biosynthesis and plant development but participate similarly in ascorbate biosynthesis. GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of the present study was to investigate the respective role in ascorbate and cell wall biosynthesis of the two SlGME genes present in tomato by targeting each of them through an RNAi-silencing approach. Taken individually SlGME1 and SlGME2 allowed normal ascorbate accumulation in the leaf and fruits, thus suggesting the same function regarding ascorbate. However, SlGME1 and SlGME2 were shown to play distinct roles in cell wall biosynthesis, depending on the tissue considered. The RNAi-SlGME1 plants harbored small and poorly seeded fruits resulting from alterations of pollen development and of pollination process. In contrast, the RNAi-SlGME2 plants exhibited vegetative growth delay while fruits remained unaffected. Analysis of SlGME1- and SlGME2-silenced seeds and seedlings further showed that the dimerization state of pectin rhamnogalacturonan-II (RG-II) was altered only in the RNAi-SlGME2 lines. Taken together with the preferential expression of each SlGME gene in different tomato tissues, these results suggest sub-functionalization of SlGME1 and SlGME2 and their specialization for cell wall biosynthesis in specific tomato tissues. |
| Databáze: | OpenAIRE |
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