The Possible Anti-Inflammatory Effect of Dehydrocostus Lactone on DSS-Induced Colitis in Mice
| Autor: | Zongqi He, Tuo Chen, Yugen Chen, Bensheng Wu, Qing Zhou, Qiong Wang, Zhao-feng Shen, Hong-tao Shang, Wei-xin Zhang, Shutang Han |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Article Subject
medicine.drug_class Inflammation Pharmacology Anti-inflammatory Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound Other systems of medicine 0302 clinical medicine Mesalazine Oral administration medicine Colitis Barrier function 030304 developmental biology 0303 health sciences business.industry medicine.disease Ulcerative colitis Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis medicine.symptom business RZ201-999 Research Article |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2020 (2020) Evidence-based Complementary and Alternative Medicine : eCAM |
| ISSN: | 1741-4288 |
| Popis: | Background. Dehydrocostus lactone (DL), one of the main active constituents in Aucklandia lappa Decne. (Muxiang), reported to have anti-inflammatory, antiulcer, and immunomodulatory properties. However, the effect of DL on ulcerative colitis (UC) has not been reported. To analyze the anti-inflammatory potential role of DL in UC, we provide a mechanism for the pharmacological action of DL. Methods. The experimental model of UC was induced by using oral administration of 2% dextran sulfate sodium (DSS) with drinking water in BALB/c mice. Mesalazine (Mes, 0.52 g/kg/d), DL-high doses (DL-H, 20 mg/kg/d), DL-middle doses (DL-M, 15 mg/kg/d), DL-low doses (DL-L, 10 mg/kg/d) were gavaged once a day from day 4 to day 17. Disease activity index (DAI) was calculated daily. On day 18, mice were rapidly dissected and the colorectal tissues were used to detect the levels of UC-related inflammatory cytokines (TNF-α, IL-1β, MCP-1, MPO, SOD, IL-6, IL-17, and IL-23), IL-6/STAT3 inflammatory signaling pathway (iNOS, COX2, IL-6, GP130, L-17, and IL-23), and colorectal mucosal barrier-related regulatory factors (MUC2, XBP1s, and α-defensins) by ELISA or qRT-PCR. Results. DL reduced the colorectal inflammation histological assessment, decreased UC-related inflammatory cytokines (TNF-α, IL-1β, MCP-1, MPO, SOD, IL-6, IL-17, and IL-23), downregulated IL-6/STAT3 inflammatory signaling pathway (iNOS, COX2, IL-6, GP130, L-17, and IL-23), repaired the key colorectal mucosal barrier protein-MUC2, and inhibited the downstream pathway (XBP1s and α-defensin). Conclusions. DL possessed the potential of anti-inflammatory effect to treated colitis. The protective mechanism of DL may involve in reducing inflammation and improving colorectal barrier function via downregulating the IL-6/STAT3 signaling. |
| Databáze: | OpenAIRE |
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