Protective Effects of Manassantin A against Ethanol-Induced Gastric Injury in Rats
| Autor: | Jong-Keun Son, Young-Suk Won, Og-Sung Moon, Ji-Won Song, Hwa-Young Son, Tae-In Kim, Chang-Seob Seo, Hyo-Jung Kwon |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Antioxidant medicine.medical_treatment Stomach Diseases Pharmaceutical Science Pharmacology Lignans Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Malondialdehyde Saururaceae Gastric mucosa Animals Medicine Prostaglandin E2 Ethanol Molecular Structure biology Superoxide Dismutase business.industry Interleukin General Medicine Glutathione Anti-Ulcer Agents Catalase Rats Nitric oxide synthase 030104 developmental biology medicine.anatomical_structure chemistry Immunology biology.protein Cyclooxygenase business Omeprazole medicine.drug |
| Zdroj: | Biological & Pharmaceutical Bulletin. 39:221-229 |
| ISSN: | 1347-5215 0918-6158 |
| Popis: | Manassantin A, a neolignan isolated from Saururus chinensis, is a major phytochemical compound that has various biological activities, including anti-inflammatory, neuroleptic, and human acyl-CoA : cholesterol acyltransferase (ACAT) inhibitory activities. In this study, we investigated the protective effects of manassantin A against ethanol-induced acute gastric injury in rats. Gastric injury was induced by intragastric administration of 5 mL/kg body weight of absolute ethanol to each rat. The positive control group and the manassantin A group were given oral doses of omeprazole (20 mg/kg) or manassantin A (15 mg/kg), respectively, 1 h prior to the administration of absolute ethanol. Our examinations revealed that manassantin A pretreatment reduced ethanol-induced hemorrhage, hyperemia, and epithelial cell loss in the gastric mucosa. Manassantin A pretreatment also attenuated the increased lipid peroxidation associated with ethanol-induced acute gastric lesions, increased the mucosal glutathione (GSH) content, and enhanced the activities of antioxidant enzymes. The levels of pro-inflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β were clearly decreased in the manassantin A-pretreated group. In addition, manassantin A pretreatment enhanced the levels of cyclooxygenase (COX)-1, COX-2, and prostaglandin E2 (PGE2) and reduced the inducible nitric oxide synthase (iNOS) overproduction and nuclear factor kappa B (NF-κB) phosphorylation. Collectively, these results indicate that manassantin A protects the gastric mucosa from ethanol-induced acute gastric injury, and suggest that these protective effects might be associated with COX/PGE2 stimulation, inhibition of iNOS production and NF-κB activation, and improvements in the antioxidant and anti-inflammatory status. |
| Databáze: | OpenAIRE |
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