Development, in vitro biocompatibility, and antitumor efficacy of acetic acid-modified Cordyceps sinensis polysaccharide nanoparticle drug delivery system
| Autor: | Nian-Qiu Shi, Jiao Guan, Junmin Wang, He-Yun Zhu, Liqin Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biocompatibility
Nanoparticle Docetaxel Polysaccharide 030226 pharmacology & pharmacy 01 natural sciences 03 medical and health sciences Acetic acid chemistry.chemical_compound 0302 clinical medicine Pharmacy and materia medica Zeta potential Cordyceps sinensis polysaccharide chemistry.chemical_classification Cordyceps biology biology.organism_classification In vitro 0104 chemical sciences RS1-441 010404 medicinal & biomolecular chemistry chemistry Antitumor efficacy Drug delivery Nanoparticles Nuclear chemistry |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Vol 56 (2020) Brazilian Journal of Pharmaceutical Sciences, Volume: 56, Article number: e18470, Published: 07 DEC 2020 Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18470 Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18470 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 2175-9790 1984-8250 |
| Popis: | Docetaxel-loaded acetic acid conjugated Cordyceps sinensis polysaccharide (DTX-AA-CSP) nanoparticles were prepared through dialysis and their release rates in vitro, particle sizes, zeta potentials, drug loading capacities, and encapsulation efficiencies were characterized for the synthesis of AA-modified CSPs from traditional Chinese medicine Cordyceps sinensis (Berk.) Sacc. Then, the AA-modified CSPs were characterized by 1 H-NMR and FT-IR. Furthermore, the biocompatibility of the delivery carrier (AA-CSP nanoparticles) was assessed on human umbilical vein endothelial cells. In vitro antitumor activity studies on DTX-AA-CSP nanoparticles were conducted on the human liver (HepG2) and colon cancer cells (SW480). The DTX-AA-CSP nanoparticles were spherical and had an average size of 98.91±0.29 nm and zeta potential within the −19.75±1.13 mV. The encapsulation efficiency and loading capacity were 80.95%±0.43% and 8.09%±0.04%, respectively. In vitro, DTX from the DTX-AA-CSP nanoparticles exhibited a sustained release, and the anticancer activities of DTX-AA-CSP nanoparticles against SW480 and HepG2 were significantly higher than those of marketed docetaxel injection (Taxotere®) in nearly all the tested concentrations. The AA-CSP nanoparticles showed good biocompatibility. This study provided a promising biocompatible delivery system for carrying antitumor drugs for cancer therapy. |
| Databáze: | OpenAIRE |
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