Adenovirus transformation revenant resistant to retransformation by E1 but not by SV40-T and HPV16-E7 oncogenes
| Autor: | Mounir Diouri, Sucheta Sircar, Joseph M. Weber, Dominique Roberge, Joseph Horvath |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Antigens
Polyomavirus Transforming Papillomavirus E7 Proteins viruses Heterologous Biology medicine.disease_cause Adenoviridae Cell Line Mice Plasmid Species Specificity Virology medicine Animals Genes Suppressor Mutation Cell fusion Oncogene Proteins Viral Oncogenes Transfection Cell Transformation Viral Molecular biology Rats Transformation (genetics) Cell culture Adenovirus E1A Proteins |
| Zdroj: | Virology. 191:187-192 |
| ISSN: | 0042-6822 |
| Popis: | We have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to E1 but not to heterologous oncogenes such as c-myc, N-ras, or polyoma middle t (Sircar et al. (1988) Oncogene 3, 725–728). DNA tumor virus oncogenes have been suggested to transform cells via the common mechanism of sequestering the Rb-105 antioncoprotein. This paradigm would predict that our revertant cell line, which is resistant to retransformation by E1a, should also be resistant to the other members of the Rb-105 binding family of oncogenes. To test this hypothesis we transfected the revenant cell line with plasmids bearing SV40-T or the HPV16-E7 oncogenes. Because transformation was obtained by both oncogenes at efficiencies similar to the transformation of a related revertant cell line, the results suggest that the resistance phenotype is specific to E1a. This specificity was further confirmed by cell fusion experiments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|