Bovine seminal ribonuclease attached to nanoparticles made of polylactic acid kills leukemia and lymphoma cell lines in vitro
| Autor: | Jörg Kreuter, Klaus Langer, Jens-Uwe Vogel, Tomas Slavik, Jindrich Cinatl, Dirk Schwabe, Jaroslav Cinatl, Martin Michaelis, Josef Matoušek |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Cancer Research Lymphoma Polymers Polyesters Tetrazolium Salts Antineoplastic Agents Apoptosis Pharmacology Mice chemistry.chemical_compound Polylactic acid In vivo Endoribonucleases Testis Tumor Cells Cultured medicine Animals Humans Cytotoxic T cell Pharmacology (medical) Lactic Acid Drug Carriers Mice Inbred ICR Leukemia Dose-Response Relationship Drug Chemistry Fibroblasts medicine.disease Spermatozoa Microspheres In vitro Thiazoles Oncology Cell culture Cytarabine Cattle Cell Division medicine.drug |
| Zdroj: | Anti-Cancer Drugs. 11:369-376 |
| ISSN: | 0959-4973 |
| Popis: | Bovine seminal ribonuclease (BS-RNase) is a protein with a number of biological effects. It shows antitumoral, aspermatogenic, antiembryonic, immunosuppressive and antiviral properties. The cytotoxic effects appear to be specific for tumor cells as non-malignant cells seem to be unaffected in vitro. Unfortunately, the in vivo application of BS-RNase so far was successful only when it was administered intratumorally. Therefore, the objective of the present investigation was to improve the properties of BS-RNase by attachment to nanoparticles made of polylactic acid (PLA-NP) using an adsorption method. This preparation was tested in vitro against leukemia (MOLT-4) and lymphoma (H9) cell lines sensitive and resistant to cytarabine. No difference between the nanoparticle preparation and pure BS-RNase was found in these tests. To examine the in vivo effects, the preparations were tested for their aspermatogenic and antiembryonal efficacy compared to the pure BS-RNase as a rapid test for antitumoral activity. The aspermatogenic and antiembryonal effects were enhanced by the nanoparticle preparation. Consequently, BS-RNase loaded adsorptively to PLA-NP holds promise for the in vivo use as an antitumoral agent. Further research will investigate the efficacy of this preparations in an in vivo tumor model. |
| Databáze: | OpenAIRE |
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