Identification of plu genes and cis-acting elements of PCNA in the Drosophila genus using conservation of gene order
| Autor: | Andrew D. Renault, J.Myles Axton |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Ribosomal Proteins
Molecular Sequence Data Regulatory Sequences Nucleic Acid Species Specificity Transcription (biology) Proliferating Cell Nuclear Antigen Sequence Homology Nucleic Acid Gene Order Genetics Melanogaster Transcriptional regulation Coding region Animals Drosophila Proteins Amino Acid Sequence Gene Phylogeny Binding Sites biology Base Sequence Sequence Homology Amino Acid Intron General Medicine DNA Sequence Analysis DNA biology.organism_classification DNA-Binding Proteins Ankyrin repeat Drosophila Drosophila melanogaster Sequence Alignment Transcription Factors |
| Zdroj: | Gene. 307 |
| ISSN: | 0378-1119 |
| Popis: | In Drosophila melanogaster, the cell cycle control gene, plutonium (plu), is located between the PCNA and RpS18 genes at position 56F on chromosome arm 2R. We have used a comparative genomic approach to investigate the evolution of the plu gene and to locate conserved cis-acting elements for plu, RpS18 and PCNA. Using primers within coding regions of PCNA and RpS18, we amplified and sequenced the intervening region from twelve Drosophila species. In each species, this region contains a plu gene resembling the D. melanogaster gene in size and in the number and position of introns. The predicted Plu sequence from the different species demonstrates that the first two ankyrin repeats are conserved. Of the transcriptional control elements of D. melanogaster PCNA, we found that three motifs 5' to the PCNA transcription unit are conserved in Drosophila species. |
| Databáze: | OpenAIRE |
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