Differences in the coronal proteome acquired by particles depositing in the lungs of asthmatic versus healthy humans
| Autor: | Lea Ann Dailey, Kenneth A. Dawson, Jonny Eriksson, Matthew Arno, Graham Somers, Paul E Pfeffer, Abhinav Kumar, Marco P. Monopoli, Elif Melis Bicer, Dave Hassall, Steven Lynham, Annelie F. Behndig, Anders Blomberg, Ian Mudway, Katarina Edwards, Ben Forbes |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Proteome Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Biology 03 medical and health sciences Pulmonary surfactant Administration Inhalation medicine Humans General Materials Science Lung Asthma Inhalation Lactoferrin 021001 nanoscience & nanotechnology medicine.disease Silicon Dioxide 030104 developmental biology medicine.anatomical_structure Targeted drug delivery Immunology biology.protein Molecular Medicine Nanoparticles Protein Corona 0210 nano-technology Respiratory tract |
| Zdroj: | Kumar, A, Bicer, E M, Pfeffer, P, Monopoli, M P, Dawson, K A, Eriksson, J, Edwards, K, Lynham, S, Arno, M, Behndig, A F, Blomberg, A, Somers, G, Hassall, D, Dailey, L A, Forbes, B & Mudway, I 2017, ' Differences in the coronal proteome acquired by particles depositing in the lungs of asthmatic versus healthy humans ', Nanomedicine-Nanotechnology Biology And Medicine, vol. 13, no. 8, pp. 2517-2521 . https://doi.org/10.1016/j.nano.2017.06.008 |
| ISSN: | 1549-9642 |
| Popis: | Most inhaled nanomedicines in development are for the treatment of lung disease, yet little is known about their interaction with the respiratory tract lining fluids (RTLF). Here we combined the use of nano-silica, as a protein concentrator, with label-free snapshot proteomics (LC-MS/MS; key findings confirmed by ELISA) to generate a quantitative profile of the RTLF proteome and provided insight into the evolved corona; information that may be used in future to improve drug targeting to the lungs by inhaled medicines. The asthmatic coronal proteome displayed a reduced contribution of surfactant proteins (SP-A and B) and a higher contribution of α1-antitrypsin. Pathway analysis suggested that asthmatic RTLFs may also be deficient in proteins related to metal handling (e.g. lactoferrin). This study demonstrates how the composition of the corona acquired by inhaled nanoparticles is modified in asthma and suggests depressed mucosal immunity even in mild airway disease. |
| Databáze: | OpenAIRE |
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