Down-regulation of the mineralocorticoid receptor by dexamethasone in an amphibian kidney cell line (A6)
| Autor: | Rodney D. Hagley, Charles O. Watlington |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.drug_class Xenopus Down-Regulation Biology Kidney Dexamethasone Epithelium Substrate Specificity Endocrinology Mineralocorticoid receptor Internal medicine medicine Animals Receptor Aldosterone Cells Cultured Mineralocorticoid receptor binding Dose-Response Relationship Drug General Medicine Dose–response relationship Receptors Mineralocorticoid Mineralocorticoid Cell culture hormones hormone substitutes and hormone antagonists Glucocorticoid medicine.drug |
| Zdroj: | Endocrine research. 22(3) |
| ISSN: | 0743-5800 |
| Popis: | The A6 cell line, derived from Xenopus kidney, is an in vitro model of cortico-steroid mediated transepitheial Na+ transport stimulation. We report the apparent down-regulation of mineralocorticoid receptor levels in A6 cells, in response to the presence of the synthetic glucorticoid dexamethasone in the culture medium. Mineralocorticoid receptor binding was suppressed to approximately 25% of control following 24-hour exposure to 10nM dexamethasone. Scatchard analysis of concentration-binding experiments show down-regulation of maximum binding capacity by Dex exposure with no alteration of MR affinity, i.e., alteration of MR number only. The effect is dose-responsive with half-maximal down regulation at 1nM. Maximal inhibition of binding occurred after 24-hours exposure to dexamethasone. The inhibitory effect of dexamethasone on MR binding was unique for the glucocorticoid, with no effect exhibited following similar treatment with an androgen, an estrogen, or a mineralocorticoid. |
| Databáze: | OpenAIRE |
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