Genome-wide transcriptomic analysis of the forebrain of postnatal Slc13a4
Autor: | Michael Piper, David G. Simmons, Sazia Sharmin, Raul Ayala Davila, Tracey J. Harvey, Diana Vidovic |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Science (General) QH301-705.5 RNA-sequencing Biology Data Note General Biochemistry Genetics and Molecular Biology Transcriptome 03 medical and health sciences Q1-390 Mice 0302 clinical medicine Sulfation Prosencephalon Downregulation and upregulation Animals Biology (General) Gene Symporters Gene Expression Profiling RNA Transporter General Medicine Sulfate Cell biology 030104 developmental biology Sulfate Transporters Forebrain Medicine Slc13a4 030217 neurology & neurosurgery Function (biology) |
Zdroj: | BMC Research Notes BMC Research Notes, Vol 14, Iss 1, Pp 1-4 (2021) |
ISSN: | 1756-0500 |
Popis: | Objective Sulfation is an essential physiological process that regulates the function of a wide array of molecules involved in brain development. We have previously shown expression levels for the sulfate transporter Slc13a4 to be elevated during postnatal development, and that sulfate accumulation in the brains of Slc13a4+/− mice is reduced, suggesting a role for this transporter during this critical window of brain development. In order to understand the pathways regulated by cellular sulfation within the brain, we performed a bulk RNA-sequencing analysis of the forebrain of postnatal day 20 (P20) Slc13a4 heterozygous mice and wild-type litter mate controls. Data description We performed an RNA transcriptomic based sequencing screen on the whole forebrain from Slc13a4+/− and Slc13a4+/+mice at P20. Differential expression analysis revealed 90 differentially regulated genes in the forebrain of Slc13a4+/− mice (a p-value of 0.1 was considered as significant). Of these, 55 were upregulated, and 35 were downregulated in the forebrain of heterozygous mice. Moreover, when we stratified further with a ± 1.2 fold-change, we observed 38 upregulated, and 16 downregulated genes in the forebrain of heterozygous mice. This resource provides a useful tool to interrogate which pathways may require elevated sulfate levels to drive normal postnatal development of the brain. |
Databáze: | OpenAIRE |
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