Expression and Capping of a Proliferation-Associated Surface Membrane p34 kDa Antigen on Different Human Hematopoietic Cell Lines
| Autor: | John L. Pauly, Edward L. Hurley, Richard J. Streck |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.drug_class T-Lymphocytes Immunology Biology Endocytosis Monoclonal antibody Cell Line Cell cycle phase Antigen Leukocytes medicine Humans Immunology and Allergy Patching and Capping B-Lymphocytes Leukemia Cell growth Chromosomes Human Pair 11 Cell Cycle Cell Membrane Antibodies Monoclonal Cell Biology Immunogold labelling Flow Cytometry Molecular biology Hematopoiesis Microscopy Fluorescence Cell culture Antigens Surface Microscopy Electron Scanning Cell Division |
| Zdroj: | Journal of Leukocyte Biology. 50:182-191 |
| ISSN: | 1938-3673 0741-5400 |
| DOI: | 10.1002/jlb.50.2.182 |
| Popis: | A novel surface membrane nonglycosylated acidic polypeptide (34 kDa), encoded by a structural gene on chromosome 11, has been identified using murine monoclonal antibody (MoAb) 53.6 (lgG2a). MoAb 53.6, raised against uninduced cells of a human erythroleukemia line (HEL), recognizes a surface membrane antigen that is displayed on proliferating (cell cycle phase G., S, and M + G2 phase) human leukocytes. The expression and redistribution (i.e., patching and capping) of the p34 kDa antigen on 27 different long-term human hematopoietic cell (HHC) lines was defined by fluorescence microscopy. These lines had been established from patients with leukemia or healthy donors and included phenotypically defined populations of T cells, B cells, and myelomonocytic cells. Almost all (>95%) of the leukocytes of the 27 lines reacted strongly with MoAb 53.6. The majority of the leukocytes displayed p34 kDa antigen patching (26/27 lines; patched cells, 96–100%); moreover, 20 of 27 lines exhibited p34 kDa antigen capping (capped cells, 8–96%). Presentation of the p34 kDa antigen on surface membrane ultrastructures, imaged with immunogold using an indirect antibody labeling procedure, was illustrated by scanning electron microscopy, and endocytosis of the gold-tagged antigen-antibody complex was studied by transmission electron microscopy. The HHC lines are thought to represent immortalized populations of different human leukocyte subsets that are in different stages of maturation and/or differentiation; thus these lines should prove useful as models for further characterizing this unique p34 kDa proliferation-associated antigen and for defining the mechanisms and significance of surface membrane antigen redistribution and modulation that has been associated with leukocyte activation and propagation. |
| Databáze: | OpenAIRE |
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