| Autor: | Hillert, Reyk, Gieseler, Anne, Krusche, Andreas, Humme, Daniel, Röwert-Huber, Hans-Joachim, Sterry, Wolfram, Walden, Peter, Schubert, Walter |
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| Předmět: |
CD4-Positive T-Lymphocytes
Keratinocytes 0301 basic medicine Pathology medicine.medical_specialty Skin Neoplasms T cell Fluorescent Antibody Technique Human skin CD8-Positive T-Lymphocytes Biology Models Biological Article 03 medical and health sciences Immune system T-Lymphocyte Subsets Tumor Microenvironment medicine Humans Cytotoxic T cell Multidisciplinary Epidermis (botany) Cell biology 030104 developmental biology medicine.anatomical_structure Antigens Surface Basal lamina Keratinocyte Biomarkers CD8 |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Immune surveillance of tumour cells is an important function of CD8 T lymphocytes, which has failed in cancer for reasons still unknown in many respect but mainly related to cellular processes in the tumour microenvironment. Applying imaging cycler microscopy to analyse the immune contexture in a human skin cancer we could identify and map 7,000 distinct cell surface-associated multi-protein assemblies. The resulting combinatorial geometry-based high-functional resolution led to discovery of a mechanism of T cell trapping in the epidermis, which involves SPIKE, a network of suprabasal keratinocyte projections piercing and interconnecting CD8 T cells. It appears initiated by clusters of infrabasal T and dendritic cells connected via cell projections across a fractured basal lamina to suprabasal keratinocytes and T lymphocytes. |
| Databáze: | OpenAIRE |
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