Coimmobilization of urease and glutamate dehydrogenase in electrochemically prepared polypyrrole-polyvinyl sulfonate films
| Autor: | Manju Gerard, Ashok Mulchandani, Bansi D. Malhotra, Anamika Gambhir |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Polymers
Inorganic chemistry Bioengineering Biosensing Techniques Polypyrrole Electrochemistry Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Adsorption Glutamate Dehydrogenase Spectroscopy Fourier Transform Infrared Pyrroles Fourier transform infrared spectroscopy Molecular Biology Dose-Response Relationship Drug Glutamate dehydrogenase General Medicine Hydrogen-Ion Concentration Urease Kinetics Sulfonate chemistry Microscopy Electron Scanning Polyvinyls Cyclic voltammetry Sulfonic Acids Biosensor Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 96(1-3) |
| ISSN: | 0273-2289 |
| Popis: | Immobilization of urease and glutamate dehydrogenase enzymes in electrochemically prepared polypyrrole-polyvinyl sulfonate films (PPY-PVS) was carried out using physical adsorption and electrochemical entrapment techniques. Detailed studies on optimum pH, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy of the enzymes in the immobilized state were conducted. The value of the apparent Michaelis-Menten constant was experimentally determined to be 2.5 and 2.7 for physically adsorbed and electrochemically entrapped urease in PPY-PVS films, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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