Peptidoglycan from Staphylococcus aureus induces tissue factor expression and procoagulant activity in human monocytes
| Autor: | Eva Mattsson, Heiko Herwald, Lars Björck, Arne Egesten |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
medicine.disease_cause
Monocytes chemistry.chemical_compound Leukocytes Thromboplastin Lymphocytes physiology [Monocytes] Non-U.S. Gov't Host Response and Inflammation biosynthesis [Thromboplastin] pharmacology [Peptidoglycan] metabolism [Peptidoglycan] pharmacology [Coagulants] microbiology [Mononuclear] Blood Coagulation Factors drug effects [Monocytes] Infectious Diseases medicine.anatomical_structure Staphylococcus aureus metabolism [Lymphocytes] medicine.symptom Support Human Immunology Inflammation Peptidoglycan microbiology [Monocytes] Biology Microbiology Peripheral blood mononuclear cell Proinflammatory cytokine Microbiology in the medical area Tissue factor metabolism [Coagulants] drug effects [Mononuclear] medicine Cell Adhesion Humans physiology [Mononuclear] Coagulants Monocyte metabolism [Staphylococcus aureus] chemistry cytology [Mononuclear] Leukocytes Mononuclear Parasitology |
| Zdroj: | Infection and Immunity; 70(6), pp 3033-3039 (2002) |
| ISSN: | 1098-5522 |
| Popis: | Staphylococcus aureus is one of the most significant pathogens in human sepsis and endocarditis. S. aureus can initiate blood coagulation, leading to the formation of microthrombi and multiorgan dysfunction in sepsis, whereas in endocarditis the bacterium induces fibrin clots on the inner surface of the heart, so-called endocardial vegetations. In the present study, we show that live and heat-killed S. aureus bacteria are potent inducers of procoagulant activity in human peripheral blood mononuclear cells. Furthermore, purified peptidoglycan, the main cell wall component of S. aureus , induced procoagulant activity in mononuclear cells in a concentration-dependent fashion. The procoagulant activity in these cells was dependent on expression of tissue factor, since antibodies to tissue factor inhibited the effect of peptidoglycan. In mononuclear cells stimulated with peptidoglycan, reverse transcription-PCR showed tissue factor gene expression, and the gene product was detected by enzyme-linked immunosorbent assay. Finally, flow cytometry identified tissue factor at the surface of CD14-positive monocytes. Peptidoglycan is known to induce proinflammatory cytokine production in monocytes. The present investigation shows that peptidoglycan also activates the extrinsic pathway of coagulation by inducing the expression of tissue factor in these cells. This mechanism helps to explain the procoagulant activity, which plays such an important role in the pathogenicity of severe S. aureus infections. |
| Databáze: | OpenAIRE |
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