An efficient gene disruption system for the nematophagous fungus Purpureocillium lavendulum
Autor: | Jing-Jing Han, Ke-Qin Zhang, Yan-Ru Cao, Zhi-Yi Guo, He-Yu Yang, Lianming Liang, Jianping Xu, Hai-Feng Fan, Chen Mi, Chen-Chen Zhang, Lu Liu |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
Genetics Microbial Genetic Vectors Fungus 01 natural sciences Nematophagous fungus 03 medical and health sciences Purpureocillium lilacinum Gene Knockout Techniques Plasmid Genetics Selection Genetic Homologous Recombination Transcription factor Gene Molecular Biology Ecology Evolution Behavior and Systematics Gene knockout 030304 developmental biology 0303 health sciences biology biology.organism_classification Infectious Diseases Hypocreales Homologous recombination 010606 plant biology & botany Plasmids |
Zdroj: | Fungal biology. 123(4) |
ISSN: | 1878-6146 |
Popis: | The fungus Purpureocillium lavendulum (formally Paecilomyces lilacinus) is a natural enemy of insects and plant-parasitic nematodes, and has been used as an important bio-control agent against agricultural pests all over the world. In order to understand the genetic mechanisms governing its biocontrol efficiency and other biological processes, an effective gene disruption system is needed. Here we report the development of an efficient system which integrates selective markers that differ from Purpureocillium lilacinum, a one-step construction method for gene knockout plasmids, and a ku80 knockout strain for efficient homologous recombination. With this system, we effectively disrupted the transcription factors in the central regulation pathway of sporulation and a serine protease which were contributed to nematode infection, demonstrating this system as an efficient gene disrupting system for further characterization of genes involved in the development and pathogenesis of this fungus. |
Databáze: | OpenAIRE |
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