Oxygen consumption rate of tumour spheroids during necrotic-like core formation
| Autor: | Takato Terai, Takashi Miura, Hitoshi Shiku, Yuji Nashimoto, Kaoru Hiramoto, Ryuji Yokokawa, Kosuke Ino, Rei Mukomoto, Takuto Imaizumi |
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| Rok vydání: | 2020 |
| Předmět: |
Culture plates
Poor prognosis chemistry.chemical_element 02 engineering and technology Biochemistry Oxygen Analytical Chemistry 03 medical and health sciences Scanning electrochemical microscopy Necrosis Oxygen Consumption Core formation Neoplasms Spheroids Cellular Tumour spheroid Electrochemistry Environmental Chemistry Humans Spectroscopy 030304 developmental biology 0303 health sciences Oxygen metabolism Spheroid 021001 nanoscience & nanotechnology Cell Hypoxia chemistry embryonic structures Biophysics 0210 nano-technology |
| Zdroj: | The Analyst. 145(19) |
| ISSN: | 1364-5528 |
| Popis: | Hypoxia is one of the major hallmarks of solid tumours and is associated with the poor prognosis of various cancers. A multicellular aggregate, termed a spheroid, has been used as a tumour model with a necrotic-like core for more than 45 years. Oxygen metabolism in spheroids has been studied using phosphorescence quenching and oxygen-sensitive electrodes. However, these conventional methods require chemical labelling and physical insertion of the electrode into each spheroid, which may be functionally and structurally disruptive. Scanning electrochemical microscopy (SECM) can non-invasively analyse oxygen metabolism. Here, we used SECM to investigate whether the changes of the internal structure of spheroids affect the oxygen metabolism. We investigated the oxygen consumption rate (OCR) of MCF-7 breast tumour spheroids with and without a necrotic-like core. A numerical simulation was used to describe a method for estimating the OCR of spheroids that settled at the bottom of the conventional culture plates. The OCR per spheroid volume decreased with increasing spheroid radius, indicating the limitation of the oxygen supply to the core of the MCF-7 spheroid. Formation of the necrotic-like core did not affect the oxygen metabolism significantly, implying that the core had minimal contribution to the OCR even before necrosis occurred. OCR analysis using SECM non-invasively monitors the change of oxygen metabolism in tumour spheroids. The approach is promising to evaluate various three-dimensional culture models. |
| Databáze: | OpenAIRE |
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