Urine peptidomic biomarkers for diagnosis of patients with systematic lupus erythematosus
| Autor: | Martin Pejchinovski, Harald Mischak, L. C. Burkly, Justyna Siwy, Ru Wei, Michelle Petri, William Mullen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Proteome Urinary system Lupus nephritis Urine Disease Gastroenterology Mass Spectrometry Article 03 medical and health sciences Rheumatology Internal medicine medicine Humans Lupus Erythematosus Systemic Biomarker discovery Lupus erythematosus Receiver operating characteristic business.industry Electrophoresis Capillary medicine.disease 3. Good health 030104 developmental biology Case-Control Studies Immunology Biomarker (medicine) business Peptides Biomarkers |
| Zdroj: | Lupus. 27(1) |
| ISSN: | 1477-0962 0961-2033 |
| Popis: | BackgroundSystematic lupus erythematosus (SLE) is characterized with various complications which can cause serious organ damage in the human body. Despite the significant improvements in disease management of SLE patients, the non-invasive diagnosis is entirely missing. In this study, we used urinary peptidomic biomarkers for early diagnosis of disease onset to improve patient risk stratification, vital for effective drug treatment.MethodsUrine samples from patients with SLE, lupus nephritis (LN) and healthy controls (HCs) were analyzed using capillary electrophoresis coupled to mass spectrometry (CE-MS) for state-of-the-art biomarker discovery.ResultsA biomarker panel made up of 65 urinary peptides was developed that accurately discriminated SLE without renal involvement from HC patients. The performance of the SLE-specific panel was validated in a multicentric independent cohort consisting of patients without SLE but with different renal disease and LN. This resulted in an area under the receiver operating characteristic (ROC) curve (AUC) of 0.80 ( p ConclusionsA laboratory-based urine test was successfully established for early diagnosis of SLE patients. Our approach determined the activity of several proteases and provided novel molecular information that could potentially influence treatment efficacy. |
| Databáze: | OpenAIRE |
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