Comparative Studies of In Vitro Regeneration Capacity in Some Breeding Forms of Prunus persica (L.) Batsch
| Autor: | Irina Mitrofanova, Anatoliy Smykov, O.V. Mitrofanova, S.V. Chelombit, N.P. Lesnikova-Sedoshenko |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
photoperiodism Environmental Engineering Plant propagation lcsh:QP1-981 Regeneration (biology) lcsh:QR1-502 Organogenesis 04 agricultural and veterinary sciences Biology 01 natural sciences Industrial and Manufacturing Engineering lcsh:Microbiology lcsh:Physiology Prunus Light intensity Horticulture Callus lcsh:Zoology 040103 agronomy & agriculture 0401 agriculture forestry and fisheries lcsh:QL1-991 010606 plant biology & botany Plant stem |
| Zdroj: | BIO Web of Conferences, Vol 24, p 00055 (2020) |
| ISSN: | 2117-4458 |
| Popis: | Peach [Prunus persica (L.) Batsch] is one of the most important stone fruit crops in the world. Preservation of valuable genotypes and creation of new breeding forms need the effective methods for plant propagation. Biotechnological method makes it possible to multiply valuable genotypes in vitro and produce high-quality plant material. Plantlets were obtained from hybrid peach embryos in five cross combinations. The induction of morphogenesis and the studies of regenerative capacity were carried out on culture media Murashige, Skoog (MS) and Gamborg, Eveleigh (B5) with vitamins and plant growth regulators. The segments of plantlets with 2-3 internodes were placed on MS and B5 media. Use of B5 medium with 0.75-1.0 mg L-1 BAP and 0.1 mg L-1 IBA induced organogenesis in the studied hybrid forms. The microshoots of the hybrid form ‘Summerglo’ × ‘Nikitskiy Podarok’ had a high regeneration capacity. In the forms ‘Persey’ × ‘Nikitskiy Podarok’ and ‘KAT 92-2210’ × ‘Nikitskiy Podarok’ low regeneration capacity was noted. An increase in BAP concentration resulted in formation of hydrated microshoots and non-morphogenic callus. It was determined that to obtain normal peach microshoots, the optimal culture parameters were a temperature of 24 ± 1oC, 16-hour photoperiod, and 37.5 μM m-2s-1 light intensity. |
| Databáze: | OpenAIRE |
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