Sugar-cane bagasse cellulose-based scaffolds promote multi-cellular interactions, angiogenesis and reduce inflammation for skin tissue regeneration
| Autor: | Honita Ramphul, Dhanjay Jhurry, Fanny Gimié, Archana Bhaw-Luximon, Jessica Andries |
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| Rok vydání: | 2020 |
| Předmět: |
Keratinocytes
Male Angiogenesis Biocompatible Materials Cell Communication 02 engineering and technology Biochemistry Mice chemistry.chemical_compound Structural Biology Skin Physiological Phenomena HaCaT Cells Skin 0303 health sciences Tissue Scaffolds General Medicine 021001 nanoscience & nanotechnology Saccharum Cell biology Female medicine.symptom 0210 nano-technology Curcumin Cell Survival Neovascularization Physiologic Inflammation 03 medical and health sciences In vivo medicine Animals Humans Regeneration Rats Wistar Cellulose Molecular Biology 030304 developmental biology Wound Healing Tissue Engineering Macrophages Regeneration (biology) Endothelial Cells Fibroblasts In vitro Rats RAW 264.7 Cells chemistry Wound healing Bagasse |
| Zdroj: | International Journal of Biological Macromolecules. 157:296-310 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.04.176 |
| Popis: | In a previous article, we reported on the physico-chemical properties of cellulose-based scaffolds derived from sugar-cane bagasse and their preliminary in vitro assessment. In view of skin tissue regeneration, we here present our findings of an extensive in vitro testing of these scaffolds using key cells involved in the wound healing cascade namely fibroblasts, keratinocytes, endothelial cells and macrophages either singly or in various combinations to mimic in vivo conditions. Inflammation was quantified using TNF-α. In vivo biocompatibility as well as wound healing potential of the scaffolds was demonstrated using Wistar rats. Finally, we discuss the effect of curcumin-loaded scaffolds on inflammation and angiogenesis in vitro and in vivo. Nanosilica extracted from sugar-cane bagasse ash was also loaded in the scaffolds and its effect on biological response was assessed. |
| Databáze: | OpenAIRE |
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