Dysregulated T helper type 1 (Th1) and Th17 responses in elderly hospitalised patients with infection and sepsis

Autor: Ana Moreno-Olivera, Thomas J. Ryan, Ashanty M. Melo, John D. Coakley, Derek G. Doherty, Eamon P. Breen, Brian O'Connell, Alhanouf I. Al-Harbi, Ross McManus
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
CD4-Positive T-Lymphocytes
Male
Pulmonology
Lymphocyte
medicine.medical_treatment
Gene Expression
CD8-Positive T-Lymphocytes
Pathology and Laboratory Medicine
Biochemistry
Monocytes
White Blood Cells
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Cytotoxic T cell
Lymphocytes
Multidisciplinary
T Cells
Receptors
Interleukin-12
Cell Differentiation
Hospitalization
medicine.anatomical_structure
Cytokine
Medicine
Female
Cellular Types
Research Article
T cell
Immune Cells
Science
Immunology
Cytotoxic T cells
Infections
Sepsis
03 medical and health sciences
Immune system
Signs and Symptoms
Diagnostic Medicine
Intensive care
DNA-binding proteins
medicine
Genetics
Humans
Gene Regulation
Aged
Blood Cells
business.industry
Biology and Life Sciences
Proteins
Cell Biology
Receptors
Interleukin
Th1 Cells
medicine.disease
Regulatory Proteins
030104 developmental biology
Gene Expression Regulation
Case-Control Studies
Respiratory Infections
Th17 Cells
business
CD8
030215 immunology
Developmental Biology
Transcription Factors
Zdroj: PLoS ONE, Vol 14, Iss 10, p e0224276 (2019)
PLoS ONE
ISSN: 1932-6203
Popis: Objective The role of Th1 and Th17 lymphocyte responses in human infection and sepsis of elderly patients has yet to be clarified. Design A prospective observational study of patients with sepsis, infection only and healthy controls. Setting The acute medical wards and intensive care units in a 1000 bed university hospital. Patients 32 patients with sepsis, 20 patients with infection, and 20 healthy controls. Patients and controls were older than 65 years of age. Patients with recognised underlying immune compromise were excluded. Methods Phenotype, differentiation status and cytokine production by T lymphocytes were determined by flow cytometry. Measurements The differentiation states of circulating CD3+, CD4+, and CD8+ T cells were characterised as naive (CD45RA+, CD197+), central memory (CD45RA-, CD197+), effector memory (CD45RA-, CD197-), or terminally differentated (CD45RA+, CD197-). Expression of IL-12 and IL-23 receptors, and the transcription factors T-bet and RORγt, was analysed in circulating T lymphocytes. Expression of interferon- γ and IL-17A were analysed following stimulation in vitro. Results CD4+ T cells from patients with infection predominantly expressed effector-memory or terminally differentiated phenotypes but CD4+ T cells from patients with severe sepsis predominantly expressed naive phenotypes (p
Databáze: OpenAIRE
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