Overexpression of both Rubisco and Rubisco activase rescues rice photosynthesis and biomass under heat stress
Autor: | Kazuma Sakoda, Ichiro Terashima, Yuchen Qu, Amane Makino, Hiroshi Fukayama, Wataru Yamori, Yuji Suzuki, Eri Kondo |
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Rok vydání: | 2021 |
Předmět: |
Chlorophyll
Thermotolerance 0106 biological sciences 0301 basic medicine Physiology Ribulose-Bisphosphate Carboxylase Biomass Plant Science Photosynthesis Zea mays 01 natural sciences 03 medical and health sciences Dry weight Gene Expression Regulation Plant Plant Proteins biology Chemistry RuBisCO Oryza Plants Genetically Modified Heat stress Heat tolerance Horticulture 030104 developmental biology biology.protein Heat-Shock Response 010606 plant biology & botany |
Zdroj: | Plant, Cell & Environment. 44:2308-2320 |
ISSN: | 1365-3040 0140-7791 |
Popis: | Global warming threatens food security by decreasing crop yields through damage to photosynthetic systems, especially Rubisco activation. We examined whether co-overexpression of Rubisco and Rubisco activase improves the photosynthetic and growth performance of rice under high temperatures. We grew three rice lines-the wild-type (WT), a Rubisco activase-overexpressing line (oxRCA), and a Rubisco- and Rubisco activase-co-overexpressing line (oxRCA-RBCS)-and analyzed photosynthesis and biomass at 25 and 40°C. Compared with the WT, the Rubisco activase content was 153% higher in oxRCA and 138% higher in oxRCA-RBCS, and the Rubisco content was 27% lower in oxRCA and similar in oxRCA-RBCS. The CO2 assimilation rate (A) of WT was lower at 40°C than at 25°C, attributable to Rubisco deactivation by heat. On the other hand, that of oxRCA and oxRCA-RBCS was maintained at 40°C, resulting in higher A than WT. Notably, the dry weight of oxRCA-RBCS was 26% higher than that of WT at 40°C. These results show that increasing the Rubisco activase content without the reduction of Rubisco content could improve yield and sustainability in rice at high temperature. This article is protected by copyright. All rights reserved. |
Databáze: | OpenAIRE |
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