Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature
| Autor: | Aaron J Simkovich, Abdelali Hannoufa, Frédéric Marsolais, Alpa Puri, Muhammad Arshad, Margaret Y. Gruber, Justin B. Renaud |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Proteomics lcsh:QH426-470 lcsh:Biotechnology Response element Biology Photosynthesis 01 natural sciences Heat stress 03 medical and health sciences Gene Expression Regulation Plant lcsh:TP248.13-248.65 Genetics Plant defense against herbivory MYB Proline LC-MS/MS Transcription factor 030304 developmental biology 2. Zero hunger miR156 0303 health sciences Abiotic stress Alfalfa fungi Temperature food and beverages Proteomic lcsh:Genetics MicroRNAs Biochemistry 010606 plant biology & botany Biotechnology Research Article Medicago sativa |
| Zdroj: | BMC Genomics BMC Genomics, Vol 21, Iss 1, Pp 1-18 (2020) |
| ISSN: | 1471-2164 |
| Popis: | Background Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 °C) for 24 h. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P 2FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa. |
| Databáze: | OpenAIRE |
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