Expression of GTP-binding protein alpha subunits in human thymocytes
| Autor: | Constantine D. Tsoukas, Sabine S. Escobar, Samuel T. Waters, Panagiotis S. Kabouridis, Jack Stanners |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Gs alpha subunit
Sequence analysis GTP-Binding Protein alpha Subunits T-Lymphocytes Clinical Biochemistry Molecular Sequence Data Gene Expression Conserved sequence GTP-Binding Proteins Sequence Homology Nucleic Acid Humans Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence Gene Conserved Sequence biology Base Sequence Infant Cell Biology General Medicine Molecular biology Thymocyte Gq alpha subunit Child Preschool biology.protein Sequence Analysis Signal Transduction |
| Zdroj: | Molecular and cellular biochemistry. 144(1) |
| ISSN: | 0300-8177 |
| Popis: | In this report, we investigate G protein alpha subunit diversity in human thymocytes, utilizing common properties shared by these genes and reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis of PCR amplified gene portions, indicate the presence of members from all four G-protein families that have been described thus far. The alpha subunit genes identified are: G alpha i1-3 and G alpha z but not G alpha o from the Gi family, G alpha s from the Gs family, G alpha 11, G alpha q, and G alpha 16 from the Gq family, and G alpha 12 and G alpha 13 from the G12 family. Also in this report we present the nucleotide and predicted amino acid sequences of the human G alpha 13 cloned from a thymocyte cDNA library. The sequence of the human G alpha 13 has not been previously reported. Comparison of this sequence with the reported murine G alpha 13 shows90% identity at the deduced amino acid sequence level. We conclude that thymocytes represent a useful experimental system for the study of G protein involvement in immune responses and lymphocyte development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink